Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   M5S54_RS09770 Genome accession   NZ_CP103544
Coordinates   2022981..2023991 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 3036     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2017981..2028991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S54_RS09740 (M5S54_09740) nudB 2019705..2020157 (+) 453 WP_077221229.1 dihydroneopterin triphosphate diphosphatase -
  M5S54_RS09745 (M5S54_09745) yebC 2020186..2020926 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  M5S54_RS09750 (M5S54_09750) ruvC 2020961..2021482 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  M5S54_RS09755 (M5S54_09755) yebB 2021484..2022086 (-) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  M5S54_RS09760 (M5S54_09760) yobI 2022157..2022222 (+) 66 WP_001386853.1 stress response small protein YobI -
  M5S54_RS09765 (M5S54_09765) ruvA 2022361..2022972 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  M5S54_RS09770 (M5S54_09770) ruvB 2022981..2023991 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  M5S54_RS09775 (M5S54_09775) znuB 2024138..2024923 (-) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  M5S54_RS09780 (M5S54_09780) znuC 2024920..2025675 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  M5S54_RS09785 (M5S54_09785) znuA 2025754..2026686 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  M5S54_RS09790 (M5S54_09790) mepM 2026702..2028024 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=622969 M5S54_RS09770 WP_000568519.1 2022981..2023991(+) (ruvB) [Escherichia coli strain 3036]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=622969 M5S54_RS09770 WP_000568519.1 2022981..2023991(+) (ruvB) [Escherichia coli strain 3036]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494