Detailed information NTG0622377

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LL045_RS05505
• Genome accession: NZ_CP086083
• Coordinates: 1108040..1109362 (-)
• Length: 440 a.a.
• NCBI ID: WP_394530549.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain EIP20A
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1103040..1114362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL045_RS05475 (LL045_05460)	-	1104182..1105150 (+)	969	WP_003130649.1	PhoH family protein	-
LL045_RS05480 (LL045_05465)	-	1105208..1105687 (+)	480	WP_394530545.1	NUDIX hydrolase	-
LL045_RS05485 (LL045_05470)	ybeY	1105839..1106327 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
LL045_RS05490 (LL045_05475)	-	1106311..1106766 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
LL045_RS05495 (LL045_05480)	-	1106780..1107358 (+)	579	WP_394530548.1	nucleotidyltransferase family protein	-
LL045_RS05500 (LL045_05485)	comFC	1107393..1107797 (-)	405	WP_230315109.1	ComF family protein	Machinery gene
LL045_RS05505 (LL045_05490)	comFA	1108040..1109362 (-)	1323	WP_394530549.1	DEAD/DEAH box helicase	Machinery gene
LL045_RS05510 (LL045_05495)	-	1109419..1110048 (+)	630	WP_104142149.1	YigZ family protein	-
LL045_RS05515 (LL045_05500)	-	1110182..1110679 (+)	498	WP_104142146.1	VanZ family protein	-
LL045_RS05520 (LL045_05505)	-	1110727..1111746 (+)	1020	WP_038601219.1	DUF475 domain-containing protein	-
LL045_RS05525 (LL045_05510)	-	1111861..1112607 (+)	747	WP_269887539.1	bifunctional metallophosphatase/5'-nucleotidase	-
LL045_RS05530 (LL045_05515)	glyQ	1112892..1113845 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50228.83 Da
• Isoelectric Point: 10.1932

>NTDB_id=622377 LL045_RS05505 WP_394530549.1 1108040..1109362(-) (comFA) [Lactococcus lactis subsp. lactis strain EIP20A]
MSTNQEKLFGRLLLKNDILQLIKSTNKISVSKIFSNFLIETTVKSILGMTSITSNKIKCNRCGTFHIKNAVKLPIGAFYC
PSCIQLGRVRSDEFLYFLPQKIFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILEK
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIAFVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAAREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=622377 LL045_RS05505 WP_394530549.1 1108040..1109362(-) (comFA) [Lactococcus lactis subsp. lactis strain EIP20A]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTAACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGATAGAAACAACGGTAAAATCAATTTTGGGGATGACTTCAATTACTT
CCAATAAAATAAAATGCAACCGCTGTGGGACTTTTCATATAAAAAATGCTGTCAAACTTCCAATTGGTGCATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTTCCACAAAAGATTTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGGGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAAA
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GCTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAGGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGGAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTGCCGCGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCACTTCTAATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAACTAAAATTTCCTAAAGAAAAAATTGCTTTCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCTGCACTGATACAAATGGCTGGACGT
GTAGGTCGTAGTCCTGAGCGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGC
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-232)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.75	90.909	0.68
comFA/cflA	Streptococcus pneumoniae Rx1	53.015	90.455	0.48
comFA/cflA	Streptococcus pneumoniae D39	53.015	90.455	0.48
comFA/cflA	Streptococcus pneumoniae R6	53.015	90.455	0.48
comFA/cflA	Streptococcus pneumoniae TIGR4	53.015	90.455	0.48
comFA/cflA	Streptococcus mitis SK321	52.513	90.455	0.475
comFA/cflA	Streptococcus mitis NCTC 12261	51.508	90.455	0.466
comFA	Bacillus subtilis subsp. subtilis str. 168	37.029	100	0.38