Detailed information NTG0622264

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LLJ08_RS01465
• Genome accession: NZ_CP086067
• Coordinates: 312006..313151 (+)
• Length: 381 a.a.
• NCBI ID: WP_014818747.1
• Uniprot ID: I4CN99
• Organism: Stutzerimonas chloritidismutans strain 6L11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 307006..318151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJ08_RS01445 (LLJ08_01445)	-	308681..309271 (-)	591	WP_041015113.1	YggT family protein	-
LLJ08_RS01450 (LLJ08_01450)	proC	309322..310146 (-)	825	WP_228208026.1	pyrroline-5-carboxylate reductase	-
LLJ08_RS01455 (LLJ08_01455)	-	310157..310846 (-)	690	WP_228208027.1	YggS family pyridoxal phosphate-dependent enzyme	-
LLJ08_RS01460 (LLJ08_01460)	pilT	310911..311945 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
LLJ08_RS01465 (LLJ08_01465)	pilU	312006..313151 (+)	1146	WP_014818747.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LLJ08_RS01470 (LLJ08_01470)	-	313148..313984 (-)	837	WP_228208028.1	TIGR03915 family putative DNA repair protein	-
LLJ08_RS01475 (LLJ08_01475)	-	313984..315195 (-)	1212	WP_042927256.1	putative DNA modification/repair radical SAM protein	-
LLJ08_RS01480 (LLJ08_01480)	-	315329..315724 (+)	396	WP_003282737.1	NINE protein	-
LLJ08_RS01485 (LLJ08_01485)	-	315802..317076 (-)	1275	WP_023446074.1	dihydroorotase	-
LLJ08_RS01490 (LLJ08_01490)	-	317076..318092 (-)	1017	WP_042927198.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42457.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=622264 LLJ08_RS01465 WP_014818747.1 312006..313151(+) (pilU) [Stutzerimonas chloritidismutans strain 6L11]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=622264 LLJ08_RS01465 WP_014818747.1 312006..313151(+) (pilU) [Stutzerimonas chloritidismutans strain 6L11]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TTATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAATCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGAGCCACCGGTA
CCGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACTCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTAATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTATG
CCGTGGCCTTCGCCGAAACTGGTCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGACGGCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCTGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACGTGCAGGGTGAAATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGATTTGCGTCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGACCAGCGTGTCTCAAGGCCTGAGCCTTGAGGTCAGTGACG
ACGATCCAGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: I4CN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388