Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LK685_RS08130 Genome accession   NZ_CP086061
Coordinates   1563847..1564464 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus sp. BC1-43     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1558847..1569464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK685_RS08095 (LK685_08100) aroE 1559505..1560347 (+) 843 WP_029317883.1 shikimate dehydrogenase -
  LK685_RS08100 (LK685_08105) yhbY 1560341..1560631 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  LK685_RS08105 (LK685_08110) - 1560643..1561212 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  LK685_RS08110 (LK685_08115) yqeK 1561202..1561762 (+) 561 WP_229762701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LK685_RS08115 (LK685_08120) rsfS 1561780..1562136 (+) 357 WP_041333759.1 ribosome silencing factor -
  LK685_RS08120 (LK685_08125) - 1562133..1562876 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  LK685_RS08125 (LK685_08130) comER 1562942..1563763 (-) 822 WP_014480313.1 late competence protein ComER -
  LK685_RS08130 (LK685_08135) comEA 1563847..1564464 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  LK685_RS08135 (LK685_08140) - 1564531..1565100 (+) 570 WP_003229978.1 ComE operon protein 2 -
  LK685_RS08140 (LK685_08145) comEC 1565104..1567434 (+) 2331 WP_229762702.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LK685_RS08145 (LK685_08150) - 1567474..1567608 (-) 135 WP_003229983.1 YqzM family protein -
  LK685_RS08150 (LK685_08155) - 1567649..1567798 (+) 150 WP_003229985.1 hypothetical protein -
  LK685_RS08155 (LK685_08160) holA 1567838..1568881 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  LK685_RS08160 (LK685_08165) rpsT 1568896..1569162 (-) 267 WP_131227637.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=622168 LK685_RS08130 WP_004398514.1 1563847..1564464(+) (comEA) [Bacillus sp. BC1-43]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=622168 LK685_RS08130 WP_004398514.1 1563847..1564464(+) (comEA) [Bacillus sp. BC1-43]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39