Detailed information NTG0622047

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LKM46_RS02145
• Genome accession: NZ_CP086016
• Coordinates: 464832..465980 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate KB-PA_3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 459832..470980

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LKM46_RS02120 (LKM46_02120)	-	460983..461279 (-)	297	WP_042167624.1	DUF167 domain-containing protein	-
LKM46_RS02125 (LKM46_02125)	-	461276..461869 (-)	594	WP_003084544.1	YggT family protein	-
LKM46_RS02130 (LKM46_02130)	proC	461880..462701 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LKM46_RS02135 (LKM46_02135)	-	462713..463405 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
LKM46_RS02140 (LKM46_02140)	pilT	463620..464654 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LKM46_RS02145 (LKM46_02145)	pilU	464832..465980 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LKM46_RS02150 (LKM46_02150)	czcD	465988..466887 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LKM46_RS02155 (LKM46_02155)	-	467041..467445 (+)	405	WP_003084560.1	NINE protein	-
LKM46_RS02160 (LKM46_02160)	-	467688..469061 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LKM46_RS02165 (LKM46_02165)	-	469058..470242 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=622047 LKM46_RS02145 WP_003100959.1 464832..465980(+) (pilU) [Pseudomonas aeruginosa isolate KB-PA_3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=622047 LKM46_RS02145 WP_003100959.1 464832..465980(+) (pilU) [Pseudomonas aeruginosa isolate KB-PA_3]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382