Detailed information NTG0617293

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: LIS75_RS12985
• Genome accession: NZ_CP085388
• Coordinates: 2645082..2647460 (-)
• Length: 792 a.a.
• NCBI ID: WP_014305491.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CMF18
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2640082..2652460

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIS75_RS12950 (LIS75_12965)	-	2641155..2641883 (+)	729	WP_003152746.1	amino acid ABC transporter ATP-binding protein	-
LIS75_RS12955 (LIS75_12970)	-	2641904..2642731 (+)	828	WP_252206587.1	transporter substrate-binding domain-containing protein	-
LIS75_RS12960 (LIS75_12975)	-	2642788..2643438 (+)	651	WP_003152744.1	amino acid ABC transporter permease	-
LIS75_RS12965 (LIS75_12980)	-	2643456..2644112 (+)	657	WP_024085646.1	amino acid ABC transporter permease	-
LIS75_RS12970 (LIS75_12985)	-	2644148..2644279 (-)	132	WP_003152741.1	YrzQ family protein	-
LIS75_RS12975 (LIS75_12990)	-	2644301..2644492 (-)	192	WP_003152738.1	hypothetical protein	-
LIS75_RS12980 (LIS75_12995)	-	2644505..2644963 (-)	459	WP_003152735.1	PRC-barrel domain-containing protein	-
LIS75_RS12985 (LIS75_13000)	recD2	2645082..2647460 (-)	2379	WP_014305491.1	ATP-dependent RecD-like DNA helicase	Machinery gene
LIS75_RS12990 (LIS75_13005)	-	2647479..2648129 (-)	651	WP_003152733.1	tetratricopeptide repeat protein	-
LIS75_RS12995 (LIS75_13010)	mnmA	2648187..2649302 (-)	1116	WP_024085647.1	tRNA 2-thiouridine(34) synthase MnmA	-
LIS75_RS13000 (LIS75_13015)	-	2649339..2650478 (-)	1140	WP_003152731.1	cysteine desulfurase family protein	-
LIS75_RS13005 (LIS75_13020)	cymR	2650497..2650913 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
LIS75_RS13010 (LIS75_13025)	-	2651108..2652376 (+)	1269	WP_003152729.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88334.01 Da
• Isoelectric Point: 4.9490

>NTDB_id=617293 LIS75_RS12985 WP_014305491.1 2645082..2647460(-) (recD2) [Bacillus velezensis strain CMF18]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILADASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDEAFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=617293 LIS75_RS12985 WP_014305491.1 2645082..2647460(-) (recD2) [Bacillus velezensis strain CMF18]
GTGGATCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAGGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTATACATTTTACGGAAAAACTGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGAATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTTGGAGACGGCGCGCTCAATAAAATTCTGGCTGATGCGTCGGTGCTTT
ATGATGTGCCGAAAATGTCAAAAAAGAAAGCGGACAAACTGGCGGGAGAGCTGCAGCGGCATCAGGGGCTTGAACAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCCATGAAAATATATCAGGCCTACGAAACGGAAACCTTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAG
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAAATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATCGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCGGAACAAAACGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGGGGACCTGGAACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGCGTATCCCTTGACCCCGGTGATTATAAAAAAGACGAGGCGTTTCCGGTG
CTGCTTGCCGCGCCGACCGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGTCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAACGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTTGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGATCTTTTGGCTTCTGAAGTGCTGCCGACCGTGCGCCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCCCATGATATGAAAAAAGGGCTGCTTCCAAAGG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAATGCGTTAAAAAAGGGGTATACCTCCAGAGATATTCAAGTGCTTGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAGGACATCATGAATCCGCCTAAAGAAAAAAGGCGGGAGCTGAAATTCGGAGATGTCGTCT
ACAGGACGGGCGATAAAATATTGCAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGGAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATTACAAGAGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTTAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCTTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• AAA domain (AAA_30) (341-548)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	89.001	99.874	0.889