Detailed information NTG0616113

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG309_RS01330
• Genome accession: NZ_CP085231
• Coordinates: 279625..280770 (+)
• Length: 381 a.a.
• NCBI ID: WP_014818747.1
• Uniprot ID: I4CN99
• Organism: Stutzerimonas chloritidismutans strain ADH2-893
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 274625..285770

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG309_RS01310 (LG309_01310)	-	276300..276890 (-)	591	WP_404396234.1	YggT family protein	-
LG309_RS01315 (LG309_01315)	proC	276941..277765 (-)	825	WP_404396237.1	pyrroline-5-carboxylate reductase	-
LG309_RS01320 (LG309_01320)	-	277776..278465 (-)	690	WP_404396239.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG309_RS01325 (LG309_01325)	pilT	278530..279564 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
LG309_RS01330 (LG309_01330)	pilU	279625..280770 (+)	1146	WP_014818747.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG309_RS01335 (LG309_01335)	-	280767..281603 (-)	837	WP_404396243.1	TIGR03915 family putative DNA repair protein	-
LG309_RS01340 (LG309_01340)	-	281603..282814 (-)	1212	WP_404396246.1	putative DNA modification/repair radical SAM protein	-
LG309_RS01345 (LG309_01345)	-	282947..283342 (+)	396	WP_003282737.1	TM2 domain-containing protein	-
LG309_RS01350 (LG309_01350)	-	283420..284694 (-)	1275	WP_404396250.1	dihydroorotase	-
LG309_RS01355 (LG309_01355)	-	284694..285710 (-)	1017	WP_023446073.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42457.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=616113 LG309_RS01330 WP_014818747.1 279625..280770(+) (pilU) [Stutzerimonas chloritidismutans strain ADH2-893]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=616113 LG309_RS01330 WP_014818747.1 279625..280770(+) (pilU) [Stutzerimonas chloritidismutans strain ADH2-893]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAATCACGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTTCGGGTCAGCGCGTTCTACCAGCGCAACCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAGGAC
CCGATCGAATTCATCCACCAGCATCAGAATTGCATCGTTACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
GGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTAATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGTCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTTAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGGCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGTGAAATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGACCTGCGTCT
TCTGATCAAGCTGGGATCGGAAACCGATGGTGAGCATCTGACCAGCGTGTCCCAGGGCCTGAGCCTTGAGGTCAGTGACG
ACGATCCAGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: I4CN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388