Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NQZ80_RS03885 Genome accession   NZ_CP102244
Coordinates   815680..817173 (+) Length   497 a.a.
NCBI ID   WP_000249326.1    Uniprot ID   -
Organism   Escherichia coli O7:H15 strain ST70     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 810680..822173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQZ80_RS03865 (NQZ80_03865) pilD 811328..812137 (+) 810 WP_000895864.1 prepilin peptidase PppA Machinery gene
  NQZ80_RS03870 (NQZ80_03870) gspS2 812203..812613 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  NQZ80_RS03875 (NQZ80_03875) gspC 812631..813590 (+) 960 WP_001366858.1 type II secretion system protein GspC -
  NQZ80_RS03880 (NQZ80_03880) gspD 813620..815680 (+) 2061 WP_000498835.1 type II secretion system secretin GspD -
  NQZ80_RS03885 (NQZ80_03885) pilB/pilB1 815680..817173 (+) 1494 WP_000249326.1 type II secretion system ATPase GspE Machinery gene
  NQZ80_RS03890 (NQZ80_03890) gspF 817173..818396 (+) 1224 WP_001173402.1 type II secretion system inner membrane protein GspF -
  NQZ80_RS03895 (NQZ80_03895) gspG 818413..818868 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  NQZ80_RS03900 (NQZ80_03900) gspH 818872..819435 (+) 564 WP_001115151.1 type II secretion system minor pseudopilin GspH -
  NQZ80_RS03905 (NQZ80_03905) gspI 819432..819803 (+) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  NQZ80_RS03910 (NQZ80_03910) gspJ 819800..820405 (+) 606 WP_001254780.1 type II secretion system minor pseudopilin GspJ -
  NQZ80_RS03915 (NQZ80_03915) gspK 820402..821379 (+) 978 WP_000633195.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55257.42 Da        Isoelectric Point: 6.0089

>NTDB_id=615941 NQZ80_RS03885 WP_000249326.1 815680..817173(+) (pilB/pilB1) [Escherichia coli O7:H15 strain ST70]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLNHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQALPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=615941 NQZ80_RS03885 WP_000249326.1 815680..817173(+) (pilB/pilB1) [Escherichia coli O7:H15 strain ST70]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGTTGATGGAAGATCTCGGTTCTGCTGAGGATTTTTTTACCCTCGCCGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGACGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAACCATGAACTGACC
GCGCAGTTACGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACAGTTGAAGACCCCATCGAATATATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGTACGCTGTGCCCCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Legionella pneumophila strain ERS1305867

43.852

86.72

0.38
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362