Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   LIO31_RS00770 Genome accession   NZ_CP085086
Coordinates   120271..120705 (+) Length   144 a.a.
NCBI ID   WP_011922561.1    Uniprot ID   -
Organism   Streptococcus suis strain Ssuis_MA6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115271..125705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIO31_RS00735 - 116020..116385 (+) 366 WP_002936568.1 DUF1033 family protein -
  LIO31_RS00740 - 116420..117363 (-) 944 Protein_113 S66 peptidase family protein -
  LIO31_RS00745 comYA 117449..118399 (+) 951 WP_011921718.1 competence type IV pilus ATPase ComGA Machinery gene
  LIO31_RS00750 comYB 118311..119348 (+) 1038 WP_256770774.1 competence type IV pilus assembly protein ComGB Machinery gene
  LIO31_RS00755 comYC 119350..119631 (+) 282 WP_011921720.1 competence type IV pilus major pilin ComGC Machinery gene
  LIO31_RS00760 comGD 119612..120019 (+) 408 WP_011921721.1 competence type IV pilus minor pilin ComGD -
  LIO31_RS00765 comYE 119991..120284 (+) 294 WP_011921722.1 competence type IV pilus minor pilin ComGE Machinery gene
  LIO31_RS00770 comGF/cglF 120271..120705 (+) 435 WP_011922561.1 competence type IV pilus minor pilin ComGF Machinery gene
  LIO31_RS00775 comGG 120683..121093 (+) 411 WP_011921724.1 competence type IV pilus minor pilin ComGG -
  LIO31_RS00780 comYH 121150..122103 (+) 954 WP_024406069.1 class I SAM-dependent methyltransferase Machinery gene
  LIO31_RS00785 - 122153..123340 (+) 1188 WP_012775473.1 acetate kinase -
  LIO31_RS00790 - 123654..124205 (+) 552 WP_012775474.1 folate family ECF transporter S component -
  LIO31_RS00795 - 124261..125517 (+) 1257 WP_012775475.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16491.85 Da        Isoelectric Point: 5.9234

>NTDB_id=615206 LIO31_RS00770 WP_011922561.1 120271..120705(+) (comGF/cglF) [Streptococcus suis strain Ssuis_MA6]
MLKSKIPAFTLLECLVVLVILSGSLLIFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSWSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=615206 LIO31_RS00770 WP_011922561.1 120271..120705(+) (comGF/cglF) [Streptococcus suis strain Ssuis_MA6]
TTGTTAAAAAGTAAAATTCCTGCTTTTACACTCTTGGAATGTTTGGTGGTTCTAGTCATTCTGTCAGGTAGCCTCTTGAT
TTTTGAAGGCTTATCCAAATTACTAGTCCAGGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGCCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGTGAA
CAGGCTTTGGCCTTTGGCAAGTCATGGTCGGATGATTTTCGGAAAACAAATGATAGAGGCCAGGGTTACCAACCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GAACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae Rx1

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae D39

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae R6

52.482

97.917

0.514
  comGF/cglF Streptococcus mitis NCTC 12261

52.985

93.056

0.493
  comYF Streptococcus mutans UA140

48.611

100

0.486
  comYF Streptococcus mutans UA159

47.917

100

0.479
  comGF/cglF Streptococcus mitis SK321

50

93.056

0.465
  comGF Lactococcus lactis subsp. cremoris KW2

45.39

97.917

0.444