Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   LIO34_RS00810 Genome accession   NZ_CP085085
Coordinates   127073..127507 (+) Length   144 a.a.
NCBI ID   WP_009909097.1    Uniprot ID   -
Organism   Streptococcus suis strain Ssuis_MA8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 122073..132507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIO34_RS00775 - 122815..123180 (+) 366 WP_002936568.1 DUF1033 family protein -
  LIO34_RS00780 - 123215..124165 (-) 951 WP_002936565.1 S66 peptidase family protein -
  LIO34_RS00785 comYA 124251..125201 (+) 951 WP_002936562.1 competence type IV pilus ATPase ComGA Machinery gene
  LIO34_RS00790 comYB 125113..126150 (+) 1038 WP_255195823.1 competence type IV pilus assembly protein ComGB Machinery gene
  LIO34_RS00795 comYC 126152..126433 (+) 282 WP_009909092.1 competence type IV pilus major pilin ComGC Machinery gene
  LIO34_RS00800 comGD 126414..126821 (+) 408 WP_009909093.1 competence type IV pilus minor pilin ComGD -
  LIO34_RS00805 comYE 126793..127086 (+) 294 WP_024390433.1 competence type IV pilus minor pilin ComGE Machinery gene
  LIO34_RS00810 comGF/cglF 127073..127507 (+) 435 WP_009909097.1 competence type IV pilus minor pilin ComGF Machinery gene
  LIO34_RS00815 comGG 127485..127895 (+) 411 WP_009909099.1 competence type IV pilus minor pilin ComGG -
  LIO34_RS00820 comYH 127952..128905 (+) 954 WP_024409299.1 class I SAM-dependent methyltransferase Machinery gene
  LIO34_RS00825 - 128955..130142 (+) 1188 WP_013730078.1 acetate kinase -
  LIO34_RS00830 - 130457..131053 (+) 597 WP_009909104.1 folate family ECF transporter S component -
  LIO34_RS00835 - 131064..132320 (+) 1257 WP_013730079.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16449.77 Da        Isoelectric Point: 5.9234

>NTDB_id=615154 LIO34_RS00810 WP_009909097.1 127073..127507(+) (comGF/cglF) [Streptococcus suis strain Ssuis_MA8]
MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSWSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=615154 LIO34_RS00810 WP_009909097.1 127073..127507(+) (comGF/cglF) [Streptococcus suis strain Ssuis_MA8]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCCTTAGTCATCCTTTCAGGTAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGCCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGTGAA
CAGGCTTTGGCCTTTGGCAAGTCATGGTCGGATGATTTTCGGAAAACAAATGATAGAGGCCAGGGTTACCAACCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

53.191

97.917

0.521
  comGF/cglF Streptococcus pneumoniae D39

53.191

97.917

0.521
  comGF/cglF Streptococcus pneumoniae R6

53.191

97.917

0.521
  comGF/cglF Streptococcus pneumoniae TIGR4

53.191

97.917

0.521
  comYF Streptococcus mutans UA140

50

100

0.5
  comGF/cglF Streptococcus mitis NCTC 12261

53.731

93.056

0.5
  comYF Streptococcus mutans UA159

49.306

100

0.493
  comGF/cglF Streptococcus mitis SK321

50.746

93.056

0.472
  comGF Lactococcus lactis subsp. cremoris KW2

46.809

97.917

0.458