Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RSAU_RS07475 Genome accession   NC_022222
Coordinates   1527264..1527761 (-) Length   165 a.a.
NCBI ID   WP_020977296.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus 6850     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1522264..1532761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSAU_RS07450 (RSAU_001401) gcvPB 1522426..1523898 (-) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  RSAU_RS07455 (RSAU_001402) gcvPA 1523891..1525237 (-) 1347 WP_020977293.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RSAU_RS07460 (RSAU_001403) gcvT 1525257..1526348 (-) 1092 WP_020977294.1 glycine cleavage system aminomethyltransferase GcvT -
  RSAU_RS07465 (RSAU_001404) - 1526507..1527031 (-) 525 WP_020977295.1 shikimate kinase -
  RSAU_RS07470 - 1527021..1527167 (-) 147 WP_001789879.1 hypothetical protein -
  RSAU_RS07475 (RSAU_001405) comGF 1527264..1527761 (-) 498 WP_020977296.1 competence type IV pilus minor pilin ComGF Machinery gene
  RSAU_RS07480 comGE 1527679..1527978 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  RSAU_RS07485 (RSAU_001406) comGD 1527965..1528411 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  RSAU_RS07490 (RSAU_001407) comGC 1528389..1528700 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RSAU_RS07495 (RSAU_001408) comGB 1528714..1529784 (-) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  RSAU_RS07500 (RSAU_001409) comGA 1529756..1530730 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RSAU_RS07505 (RSAU_001410) - 1530782..1531405 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RSAU_RS07510 (RSAU_001411) - 1531402..1531731 (-) 330 WP_020977297.1 MTH1187 family thiamine-binding protein -
  RSAU_RS07515 (RSAU_001412) - 1531731..1532717 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=61323 RSAU_RS07475 WP_020977296.1 1527264..1527761(-) (comGF) [Staphylococcus aureus subsp. aureus 6850]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIEIREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=61323 RSAU_RS07475 WP_020977296.1 1527264..1527761(-) (comGF) [Staphylococcus aureus subsp. aureus 6850]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAATTAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(69-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment