Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RSAU_RS07500 Genome accession   NC_022222
Coordinates   1529756..1530730 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus subsp. aureus 6850     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1524756..1535730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSAU_RS07460 (RSAU_001403) gcvT 1525257..1526348 (-) 1092 WP_020977294.1 glycine cleavage system aminomethyltransferase GcvT -
  RSAU_RS07465 (RSAU_001404) - 1526507..1527031 (-) 525 WP_020977295.1 shikimate kinase -
  RSAU_RS07470 - 1527021..1527167 (-) 147 WP_001789879.1 hypothetical protein -
  RSAU_RS07475 (RSAU_001405) comGF 1527264..1527761 (-) 498 WP_020977296.1 competence type IV pilus minor pilin ComGF Machinery gene
  RSAU_RS07480 comGE 1527679..1527978 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  RSAU_RS07485 (RSAU_001406) comGD 1527965..1528411 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  RSAU_RS07490 (RSAU_001407) comGC 1528389..1528700 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RSAU_RS07495 (RSAU_001408) comGB 1528714..1529784 (-) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  RSAU_RS07500 (RSAU_001409) comGA 1529756..1530730 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RSAU_RS07505 (RSAU_001410) - 1530782..1531405 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RSAU_RS07510 (RSAU_001411) - 1531402..1531731 (-) 330 WP_020977297.1 MTH1187 family thiamine-binding protein -
  RSAU_RS07515 (RSAU_001412) - 1531731..1532717 (-) 987 WP_000161315.1 ROK family glucokinase -
  RSAU_RS07520 (RSAU_001413) - 1532714..1532917 (-) 204 WP_000087561.1 YqgQ family protein -
  RSAU_RS07525 (RSAU_001414) - 1532898..1534361 (-) 1464 WP_001017867.1 rhomboid family protein -
  RSAU_RS07530 (RSAU_001415) - 1534373..1534912 (-) 540 WP_042908703.1 5-formyltetrahydrofolate cyclo-ligase -
  RSAU_RS07535 (RSAU_001416) rpmG 1535121..1535270 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=61328 RSAU_RS07500 WP_000697220.1 1529756..1530730(-) (comGA) [Staphylococcus aureus subsp. aureus 6850]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=61328 RSAU_RS07500 WP_000697220.1 1529756..1530730(-) (comGA) [Staphylococcus aureus subsp. aureus 6850]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCCTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTAATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAGGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment