Detailed information NTG0612776

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LHJ63_RS14825
• Genome accession: NZ_CP084721
• Coordinates: 3044434..3045552 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain HKU7
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3039434..3050552

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LHJ63_RS14815 (LHJ63_14815)	-	3042550..3043242 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
LHJ63_RS14820 (LHJ63_14820)	pilT	3043369..3044406 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
LHJ63_RS14825 (LHJ63_14825)	pilU	3044434..3045552 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LHJ63_RS14830 (LHJ63_14830)	fur	3045622..3046059 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LHJ63_RS14835 (LHJ63_14835)	-	3046171..3046569 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LHJ63_RS14840 (LHJ63_14840)	-	3046624..3046947 (-)	324	WP_000442587.1	RnfH family protein	-
LHJ63_RS14845 (LHJ63_14845)	-	3046947..3048014 (-)	1068	WP_002019572.1	hypothetical protein	-
LHJ63_RS14850 (LHJ63_14850)	-	3048167..3048622 (-)	456	WP_000782976.1	bacteriohemerythrin	-
LHJ63_RS14855 (LHJ63_14855)	-	3048875..3049723 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=612776 LHJ63_RS14825 WP_000347039.1 3044434..3045552(+) (pilU) [Acinetobacter baumannii strain HKU7]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=612776 LHJ63_RS14825 WP_000347039.1 3044434..3045552(+) (pilU) [Acinetobacter baumannii strain HKU7]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395