Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LF844_RS24710 Genome accession   NZ_CP084625
Coordinates   5340787..5341851 (-) Length   354 a.a.
NCBI ID   WP_151137793.1    Uniprot ID   -
Organism   Metapseudomonas lalkuanensis strain ACYW.190     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5335787..5346851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF844_RS24685 (LF844_24685) aroK 5336348..5336866 (-) 519 WP_151137780.1 shikimate kinase AroK -
  LF844_RS24690 (LF844_24690) pilQ 5336872..5338980 (-) 2109 WP_151137783.1 type IV pilus secretin PilQ Machinery gene
  LF844_RS24695 (LF844_24695) pilP 5339038..5339571 (-) 534 WP_151137785.1 type 4a pilus biogenesis lipoprotein PilP -
  LF844_RS24700 (LF844_24700) pilO 5339568..5340191 (-) 624 WP_151137788.1 type 4a pilus biogenesis protein PilO -
  LF844_RS24705 (LF844_24705) - 5340188..5340787 (-) 600 WP_151137791.1 PilN domain-containing protein -
  LF844_RS24710 (LF844_24710) pilM 5340787..5341851 (-) 1065 WP_151137793.1 pilus assembly protein PilM Machinery gene
  LF844_RS24715 (LF844_24715) - 5342033..5344471 (+) 2439 WP_404804859.1 penicillin-binding protein 1A -
  LF844_RS24720 (LF844_24720) - 5344534..5345802 (-) 1269 WP_151137799.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37819.08 Da        Isoelectric Point: 4.4526

>NTDB_id=612066 LF844_RS24710 WP_151137793.1 5340787..5341851(-) (pilM) [Metapseudomonas lalkuanensis strain ACYW.190]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALSRVISKARTGVKAAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLSAKVVDVEAYALERAYSLLSAQLGDAHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=612066 LF844_RS24710 WP_151137793.1 5340787..5341851(-) (pilM) [Metapseudomonas lalkuanensis strain ACYW.190]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTATGCCGTCGAGCCGCTCCCACCAAACGCGGTTGTCGAAAAGAACA
TCGCGGAGCTGGAGGGCGTCGGGCAGGCACTTTCCCGAGTAATCAGCAAGGCGCGTACCGGCGTCAAGGCCGCGGCAGTG
GCTGTGGCCGGTTCGGCCGTGATCACCAAGACCATCGAAATGGAAGCCGGGCTCTCCGAAGACGATCTGGAGAACCAGCT
GAAGATCGAGGCCGACCAGTACATTCCCTATCCGCTGGAAGAAGTCGCCATCGACTTCGAGGTGCAAGGTGCCTCGGCAC
GCAACCCGGAGCGGGTCGATGTGCTGCTCGCAGCCTGCCGAAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTCGCCCTG
GCCGGACTTTCCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCGCCTATTCGCTGCTTTCGGCACAGCTGGG
CGATGCTCATGACGAGCTGACCGTCGCAGTCGTCGACATTGGCGCCACCATGACGACCCTCAGCGTGCTGCACAACGGCC
GCACCATCTATACCCGCGAACAGCTCTTCGGTGGTCGTCAACTGACCGAGGAGATCCAGCGTCGCTATGGTCTTTCCGTT
GAGGAAGCTGGTCTCGCCAAGAAACAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTCTGCAACCGTTCAAGGATGC
TGTAGTCCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCAGCCGGCCAGTTCAATGATGTGGATTACATCCTCCTGG
CAGGCGGCACGGCTTCGATCCCGGATCTCGATCGCCTGATCCAGCAGAAGATCGGTACACCGACCCTGGTTGCCAATCCC
TTCGCCGATATGGCGCTGAGCAGCAAGGTCAATGCTGGTGCGCTGGCTAGCGATGCGCCTGCCCTGATGATTGCGTGTGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.46