Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LF844_RS16115 Genome accession   NZ_CP084625
Coordinates   3447199..3448407 (-) Length   402 a.a.
NCBI ID   WP_226282139.1    Uniprot ID   -
Organism   Metapseudomonas lalkuanensis strain ACYW.190     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3442199..3453407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF844_RS16095 (LF844_16095) - 3442535..3443233 (-) 699 WP_226282136.1 CoA transferase subunit A -
  LF844_RS16100 (LF844_16100) - 3443360..3444268 (+) 909 WP_151133223.1 LysR family transcriptional regulator -
  LF844_RS16105 (LF844_16105) - 3444330..3446285 (+) 1956 WP_226282137.1 acetoacetate--CoA ligase -
  LF844_RS16110 (LF844_16110) - 3446377..3447015 (-) 639 WP_226282138.1 DJ-1/PfpI family protein -
  LF844_RS16115 (LF844_16115) pilU 3447199..3448407 (-) 1209 WP_226282139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LF844_RS16120 (LF844_16120) - 3448527..3448805 (+) 279 WP_151133219.1 peptidylprolyl isomerase -
  LF844_RS16125 (LF844_16125) - 3448874..3449173 (-) 300 WP_151133218.1 hypothetical protein -
  LF844_RS16130 (LF844_16130) - 3449261..3449446 (-) 186 WP_151133217.1 hypothetical protein -
  LF844_RS16135 (LF844_16135) - 3449658..3450098 (-) 441 WP_226282140.1 CopD family copper resistance protein -
  LF844_RS16140 (LF844_16140) - 3450335..3451924 (+) 1590 WP_151133147.1 ABC-F family ATPase -
  LF844_RS16145 (LF844_16145) - 3452112..3452720 (-) 609 WP_226282141.1 FMN-dependent NADH-azoreductase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 44226.58 Da        Isoelectric Point: 6.1320

>NTDB_id=612057 LF844_RS16115 WP_226282139.1 3447199..3448407(-) (pilU) [Metapseudomonas lalkuanensis strain ACYW.190]
MDLSAMLKILATQDGSDLYLSTGAPPCAKFNGLLRPLSNEPLKAGDVARIAESIMDVEQRAEFDRELEMNLAISLPNIGR
FRINIFRQRNEVSIVARNIKLDIPRFEDLKLPEVLLKMVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTSTISDLIKRGEFHSIKEIMEKSRGLGMQTFDQAL
FDLVVEGAIPEEEAIKNADSANNLRLKLKLYREGPTAAPAPAAAAPAPVAAPSATADAAGWGLELKLEDLEEDAPEEPGP
RG

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=612057 LF844_RS16115 WP_226282139.1 3447199..3448407(-) (pilU) [Metapseudomonas lalkuanensis strain ACYW.190]
ATGGACCTCTCTGCGATGCTCAAGATCCTGGCTACCCAGGATGGTTCCGACCTCTACCTGTCCACGGGCGCGCCGCCCTG
TGCCAAGTTCAACGGCCTGCTGCGGCCGCTCAGCAACGAACCGCTGAAGGCCGGCGACGTGGCCAGGATCGCCGAGTCGA
TCATGGACGTCGAGCAGCGCGCCGAGTTCGACCGCGAACTGGAGATGAACCTGGCCATCTCGCTGCCCAATATCGGCCGC
TTCCGCATCAATATCTTCCGCCAGCGCAACGAGGTGTCCATCGTCGCGCGGAACATCAAGCTGGATATCCCGCGTTTCGA
AGACCTCAAGCTGCCGGAAGTGCTGCTCAAGATGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGTGGTACCGGTT
CCGGTAAATCCACCTCGCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAAGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAGATTCGCGACCGCGAGACCATGGAACATG
CGCTGGCCTTCGCCGACACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAAGAGCGTCGTCCGCAACTGCTCAACGACCTGGGCAATAACCTCAAGGCTTTCGTTTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGGGCGGCGGTGGAGGTGCTGTTGGGTACCTCGACCATCAGCGACCTGATCA
AGCGCGGTGAATTCCACTCCATCAAGGAAATCATGGAGAAGTCCCGTGGCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTGGTGGAGGGGGCCATTCCGGAAGAGGAGGCGATCAAGAACGCCGACTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTCTATCGCGAAGGTCCGACTGCAGCGCCCGCTCCGGCCGCAGCGGCTCCGGCACCGGTAGCCGCCCCAAGCG
CCACCGCGGATGCCGCCGGCTGGGGCCTGGAGCTGAAGCTGGAAGACCTGGAAGAAGACGCCCCGGAAGAGCCGGGGCCT
CGCGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.78

88.06

0.5
  pilU Acinetobacter baylyi ADP1

53.371

88.557

0.473
  pilU Vibrio cholerae strain A1552

53.258

87.811

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.164

95.274

0.373