Detailed information NTG0610971

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LDJ84_RS02170
• Genome accession: NZ_CP084413
• Coordinates: 456960..458105 (+)
• Length: 381 a.a.
• NCBI ID: WP_024766757.1
• Uniprot ID: -
• Organism: Pseudomonas sp. HS-18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 451960..463105

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDJ84_RS02145 (LDJ84_02145)	-	453262..453552 (-)	291	WP_024766762.1	DUF167 domain-containing protein	-
LDJ84_RS02150 (LDJ84_02150)	-	453603..454196 (-)	594	WP_024766761.1	YggT family protein	-
LDJ84_RS02155 (LDJ84_02155)	proC	454210..455040 (-)	831	WP_024766760.1	pyrroline-5-carboxylate reductase	-
LDJ84_RS02160 (LDJ84_02160)	-	455058..455747 (-)	690	WP_037013506.1	YggS family pyridoxal phosphate-dependent enzyme	-
LDJ84_RS02165 (LDJ84_02165)	pilT	455821..456855 (+)	1035	WP_024766758.1	type IV pilus twitching motility protein PilT	Machinery gene
LDJ84_RS02170 (LDJ84_02170)	pilU	456960..458105 (+)	1146	WP_024766757.1	type IV pilus ATPase PilU	Machinery gene
LDJ84_RS02175 (LDJ84_02175)	-	458175..458579 (+)	405	WP_024766756.1	NINE protein	-
LDJ84_RS02180 (LDJ84_02180)	-	458804..460177 (+)	1374	WP_226045840.1	pyridoxal-phosphate dependent enzyme	-
LDJ84_RS02185 (LDJ84_02185)	-	460174..461343 (+)	1170	WP_226045841.1	trans-sulfuration enzyme family protein	-
LDJ84_RS02190 (LDJ84_02190)	-	461568..462839 (-)	1272	WP_226045842.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42584.91 Da
• Isoelectric Point: 6.7255

>NTDB_id=610971 LDJ84_RS02170 WP_024766757.1 456960..458105(+) (pilU) [Pseudomonas sp. HS-18]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAYADSANDLRLMIKLGSETDADHLTTMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=610971 LDJ84_RS02170 WP_024766757.1 456960..458105(+) (pilU) [Pseudomonas sp. HS-18]
ATGGAATTCGAGAAGCTGTTGCGCCTGATGGTGGAAAAGGGAGCCTCCGACCTGTTCATCACAGCGGGCGTACCACCGTC
GATGAAGGTCAACGGCAAGGTGTTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAAACCGTGCTGTCGG
TGATGAACGAGCAGCAGCGTCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCGTCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
TGACCTGAAACTGCCGGAAATCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACCTCGCTGGCGGCCATGATCGGCTACCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAT
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAAGTGGGCCTGGATACCGATACCTTCGAAGT
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CCGTGGCTTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCCGCTGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGTCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAACTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTATACCCAGGGCGAGATCACCTACGAAGATGCGCTGGCCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGATCGGAAACCGATGCCGACCACCTGACCACCATGACCCAGGGCCTGACCCTGGAGATGAGCGACG
ACGATCCCAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.451	100	0.885
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	60.286	91.864	0.554
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383