Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BASU_RS05660 Genome accession   NC_022081
Coordinates   1046262..1049966 (+) Length   1234 a.a.
NCBI ID   WP_020955607.1    Uniprot ID   -
Organism   Bacillus velezensis UCMB5113     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1041262..1054966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BASU_RS05645 (BASU_1016) - 1041343..1041852 (-) 510 WP_020955604.1 ferritin family protein -
  BASU_RS05650 (BASU_1017) - 1041921..1042634 (-) 714 WP_020955605.1 YetF domain-containing protein -
  BASU_RS05655 (BASU_1018) addB 1042775..1046275 (+) 3501 WP_020955606.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BASU_RS05660 (BASU_1019) addA 1046262..1049966 (+) 3705 WP_020955607.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BASU_RS05665 (BASU_1020) sbcD 1050031..1051203 (+) 1173 WP_020955608.1 exonuclease subunit SbcD -
  BASU_RS05670 (BASU_1021) sbcC 1051200..1054592 (+) 3393 WP_020955609.1 exonuclease subunit SbcC -
  BASU_RS05675 (BASU_1022) - 1054608..1054901 (+) 294 WP_007409167.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141148.50 Da        Isoelectric Point: 5.1275

>NTDB_id=61095 BASU_RS05660 WP_020955607.1 1046262..1049966(+) (addA) [Bacillus velezensis UCMB5113]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEVWLQSFVRLYDVTEESKMEELPFYQYVKEDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDELYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSE
LLEENPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGK
DLLGALRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=61095 BASU_RS05660 WP_020955607.1 1046262..1049966(+) (addA) [Bacillus velezensis UCMB5113]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAAGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTCTTGAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCGGACAGATATACGACAGACAGGCATGATCTTGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGTATGGCTGCAAAGCTTTGTCCGCCTGTATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAGTATGTGAAAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCCTTTAAGCGGGCGAAGGCGGTAAAGG
GTGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACCGATTATTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAGCAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACGAATCTTGTGCAAGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGACCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACCGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCGGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCCATCGTCGGATGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGCTGACAGACATGATGAACTGTATCAAAAACTGAGAACCT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGACTATGCAGGCGGCATGCCCGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCCCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATCCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAA
AGATGCGCAGAGAGCTCTTATCCGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTTCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACA
GGACGCCGGGACTTTCCTCATCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGAGAATCCAAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGAGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGAAGCTATTGATATAGAAGAAATTGTCCAGTTTTTCGGAACTGAAATCGGGAAA
GACCTTCTCGGGGCGCTGCGGATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTATGAAACTGCGGACGGTCTCTATTTGCTGG
ACTATAAAACTGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTTTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.577

100

0.826

Multiple sequence alignment