Detailed information NTG0061072

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: EL271_RS01305
• Genome accession: NZ_AP018937
• Coordinates: 222329..223327 (+)
• Length: 332 a.a.
• NCBI ID: WP_001809468.1
• Uniprot ID: Q8CWU7
• Organism: Streptococcus pneumoniae strain HU-OH
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 217329..228327

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL271_RS01280 (SPOH_0228)	leuS	217373..219874 (+)	2502	WP_000011760.1	leucine--tRNA ligase	-
EL271_RS01285 (SPOH_0229)	-	220068..220301 (+)	234	Protein_237	GNAT family N-acetyltransferase	-
EL271_RS01290 (SPOH_0230)	-	220407..220860 (+)	454	Protein_238	GNAT family N-acetyltransferase	-
EL271_RS01295 (SPOH_0231)	-	220872..221288 (+)	417	WP_000628393.1	GNAT family N-acetyltransferase	-
EL271_RS01300	-	222103..222195 (-)	93	WP_000970381.1	type I toxin-antitoxin system Fst family toxin	-
EL271_RS01305 (SPOH_0232)	ruvB	222329..223327 (+)	999	WP_001809468.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
EL271_RS01310	-	223308..223880 (+)	573	Protein_242	nucleotidyltransferase family protein	-
EL271_RS01315 (SPOH_0235)	-	224111..224869 (+)	759	WP_000466719.1	isoprenyl transferase	-
EL271_RS01320 (SPOH_0236)	-	224878..225681 (+)	804	WP_000189866.1	phosphatidate cytidylyltransferase	-
EL271_RS01325 (SPOH_0237)	eeP	225703..226962 (+)	1260	WP_000900660.1	RIP metalloprotease RseP	Regulator

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37288.73 Da
• Isoelectric Point: 4.5053

>NTDB_id=61072 EL271_RS01305 WP_001809468.1 222329..223327(+) (ruvB) [Streptococcus pneumoniae strain HU-OH]
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide

• Length: 999 bp

>NTDB_id=61072 EL271_RS01305 WP_001809468.1 222329..223327(+) (ruvB) [Streptococcus pneumoniae strain HU-OH]
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGGCCTCCAGGTCTCGGGAAAACGACCATGGCCTTTGTTATTGCCAACGAACTGGGAGTCAATCTT
AAGCAGACGTCGGGTCCAGTCATTGAAAAAGCCGGAGATCTGGTAGCTATTTTGAATGAGTTAGAGCCTGGGGATGTACT
TTTTATTGATGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATAGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCCAATCCGCTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGGACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTGAATATCGCCGAAGAGCGTGAGACAG
TTGAAGATATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q8CWU7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	100	100	1
ruvB	Streptococcus pneumoniae D39	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	99.096	100	0.991
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.429	98.193	0.593
ruvB	Helicobacter pylori 26695	50.774	97.289	0.494
ruvB	Synechocystis sp. PCC 6803	49.84	94.277	0.47