Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LEL82_RS30250 Genome accession   NZ_CP084321
Coordinates   6468310..6469377 (-) Length   355 a.a.
NCBI ID   WP_087935230.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HS18-89     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 6469437..6470417 6468310..6469377 flank 60


Gene organization within MGE regions


Location: 6468310..6470417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEL82_RS30250 (LEL82_30210) pilM 6468310..6469377 (-) 1068 WP_087935230.1 type IV pilus assembly protein PilM Machinery gene
  LEL82_RS30255 (LEL82_30215) - 6469437..6470417 (+) 981 WP_064613796.1 IS5 family transposase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38070.56 Da        Isoelectric Point: 4.5405

>NTDB_id=610605 LEL82_RS30250 WP_087935230.1 6468310..6469377(-) (pilM) [Pseudomonas aeruginosa strain HS18-89]
MSLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAV
VAVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALA
LAGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLS
VEEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVAN
PFADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=610605 LEL82_RS30250 WP_087935230.1 6468310..6469377(-) (pilM) [Pseudomonas aeruginosa strain HS18-89]
ATTTCCCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGA
GTTGAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGA
ACATCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTG
GTCGCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCA
GCTGAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCG
CGCGCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCG
CTGGCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCT
GGGTGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACG
GACGCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCG
GTGGAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGA
CGCCGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCC
TGGCGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAAC
CCGTTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTG
CGGCCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.775

100

0.558
  comM Acinetobacter nosocomialis M2

55.775

100

0.558
  comM Acinetobacter baylyi ADP1

51.841

99.437

0.515
  pilM Legionella pneumophila strain ERS1305867

46.459

99.437

0.462