Detailed information NTG0610476

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LDO71_RS13965
• Genome accession: NZ_CP084297
• Coordinates: 2943646..2944764 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain LHC22-2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2938646..2949764

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO71_RS13955 (LDO71_13955)	-	2941762..2942454 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
LDO71_RS13960 (LDO71_13960)	pilT	2942581..2943618 (+)	1038	WP_225545815.1	type IV pilus twitching motility protein PilT	Machinery gene
LDO71_RS13965 (LDO71_13965)	pilU	2943646..2944764 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LDO71_RS13970 (LDO71_13970)	fur	2944834..2945271 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LDO71_RS13975 (LDO71_13975)	-	2945383..2945781 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LDO71_RS13980 (LDO71_13980)	-	2945836..2946159 (-)	324	WP_000442586.1	RnfH family protein	-
LDO71_RS13985 (LDO71_13985)	-	2946159..2947226 (-)	1068	WP_225545816.1	hypothetical protein	-
LDO71_RS13990 (LDO71_13990)	-	2947379..2947834 (-)	456	WP_000782976.1	bacteriohemerythrin	-
LDO71_RS13995 (LDO71_13995)	-	2948087..2948935 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=610476 LDO71_RS13965 WP_000347034.1 2943646..2944764(+) (pilU) [Acinetobacter baumannii strain LHC22-2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=610476 LDO71_RS13965 WP_000347034.1 2943646..2944764(+) (pilU) [Acinetobacter baumannii strain LHC22-2]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACTAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39