Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NMK62_RS02065 Genome accession   NZ_CP101310
Coordinates   429934..431172 (+) Length   412 a.a.
NCBI ID   WP_000816014.1    Uniprot ID   -
Organism   Escherichia coli strain 99-3311     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424934..436172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK62_RS02040 (NMK62_02040) mrcA 425214..427766 (-) 2553 WP_001400412.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  NMK62_RS02045 (NMK62_02045) hofM 427886..428665 (+) 780 WP_001400410.1 DNA utilization protein HofM -
  NMK62_RS02050 (NMK62_02050) hofN 428665..429204 (+) 540 WP_001069342.1 DNA utilization protein HofN -
  NMK62_RS02055 (NMK62_02055) hofO 429188..429628 (+) 441 WP_001055740.1 DNA utilization protein HofO -
  NMK62_RS02060 (NMK62_02060) hofP 429618..430022 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  NMK62_RS02065 (NMK62_02065) comE 429934..431172 (+) 1239 WP_000816014.1 DNA uptake porin HofQ Machinery gene
  NMK62_RS02070 (NMK62_02070) aroK 431573..432094 (+) 522 WP_000818618.1 shikimate kinase AroK -
  NMK62_RS02075 (NMK62_02075) aroB 432151..433239 (+) 1089 WP_000439845.1 3-dehydroquinate synthase -
  NMK62_RS02080 (NMK62_02080) damX 433331..434617 (+) 1287 WP_000343195.1 cell division protein DamX -
  NMK62_RS02085 (NMK62_02085) dam 434724..435560 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44735.24 Da        Isoelectric Point: 5.9488

>NTDB_id=610327 NMK62_RS02065 WP_000816014.1 429934..431172(+) (comE) [Escherichia coli strain 99-3311]
MKQWIAALLLMLIPGVQAVKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWAAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=610327 NMK62_RS02065 WP_000816014.1 429934..431172(+) (comE) [Escherichia coli strain 99-3311]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGTAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAAAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCCCTTGAACAGTGGGCAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-178)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  comE Haemophilus influenzae 86-028NP

36.697

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364