Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NML25_RS21835 Genome accession   NZ_CP101222
Coordinates   4509931..4511169 (-) Length   412 a.a.
NCBI ID   WP_072020699.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-144     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4504931..4516169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML25_RS21815 (NML25_21815) dam 4505542..4506378 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  NML25_RS21820 (NML25_21820) damX 4506485..4507771 (-) 1287 WP_001551753.1 cell division protein DamX -
  NML25_RS21825 (NML25_21825) aroB 4507863..4508951 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  NML25_RS21830 (NML25_21830) aroK 4509008..4509529 (-) 522 WP_000818618.1 shikimate kinase AroK -
  NML25_RS21835 (NML25_21835) comE 4509931..4511169 (-) 1239 WP_072020699.1 DNA uptake porin HofQ Machinery gene
  NML25_RS21840 (NML25_21840) hofP 4511081..4511485 (-) 405 WP_001551755.1 DNA utilization protein HofP -
  NML25_RS21845 (NML25_21845) hofO 4511475..4511915 (-) 441 WP_001551756.1 DNA utilization protein HofO -
  NML25_RS21850 (NML25_21850) hofN 4511899..4512438 (-) 540 WP_001069326.1 DNA utilization protein HofN -
  NML25_RS21855 (NML25_21855) hofM 4512438..4513217 (-) 780 WP_001551759.1 DNA utilization protein HofM -
  NML25_RS21860 (NML25_21860) mrcA 4513337..4515889 (+) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44722.24 Da        Isoelectric Point: 5.9488

>NTDB_id=609281 NML25_RS21835 WP_072020699.1 4509931..4511169(-) (comE) [Escherichia coli strain EC21Z-144]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADVGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=609281 NML25_RS21835 WP_072020699.1 4509931..4511169(-) (comE) [Escherichia coli strain EC21Z-144]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACGCTGCAATACGCCGACGTGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.071

100

0.393
  comE Haemophilus influenzae Rd KW20

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.476

100

0.362