NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	LAZ98_RS13060	Genome accession	NZ_CP083764
Coordinates	2587799..2588869 (-)	Length	356 a.a.
NCBI ID	WP_014418378.1	Uniprot ID	A0AAP4DGV9
Organism	Bacillus velezensis strain DMB07
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582799..2593869

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LAZ98_RS13010 (LAZ98_13010)	sipW	2582858..2583442 (-)	585	WP_012117977.1	signal peptidase I SipW	-
LAZ98_RS13015 (LAZ98_13015)	tapA	2583414..2584085 (-)	672	WP_014418371.1	amyloid fiber anchoring/assembly protein TapA	-
LAZ98_RS13020 (LAZ98_13020)	-	2584344..2584673 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
LAZ98_RS13025 (LAZ98_13025)	-	2584714..2584893 (-)	180	WP_003153093.1	YqzE family protein	-
LAZ98_RS13030 (LAZ98_13030)	comGG	2584950..2585327 (-)	378	WP_025649851.1	competence type IV pilus minor pilin ComGG	Machinery gene
LAZ98_RS13035 (LAZ98_13035)	comGF	2585328..2585723 (-)	396	WP_025649850.1	competence type IV pilus minor pilin ComGF	-
LAZ98_RS13040 (LAZ98_13040)	comGE	2585737..2586051 (-)	315	WP_017418140.1	competence type IV pilus minor pilin ComGE	Machinery gene
LAZ98_RS13045 (LAZ98_13045)	comGD	2586035..2586472 (-)	438	WP_007612572.1	competence type IV pilus minor pilin ComGD	Machinery gene
LAZ98_RS13050 (LAZ98_13050)	comGC	2586462..2586770 (-)	309	WP_014418376.1	competence type IV pilus major pilin ComGC	Machinery gene
LAZ98_RS13055 (LAZ98_13055)	comGB	2586775..2587812 (-)	1038	WP_014418377.1	competence type IV pilus assembly protein ComGB	Machinery gene
LAZ98_RS13060 (LAZ98_13060)	comGA	2587799..2588869 (-)	1071	WP_014418378.1	competence type IV pilus ATPase ComGA	Machinery gene
LAZ98_RS13065 (LAZ98_13065)	-	2589062..2590012 (-)	951	WP_071181848.1	magnesium transporter CorA family protein	-
LAZ98_RS13070 (LAZ98_13070)	-	2590158..2591459 (+)	1302	WP_071181849.1	hemolysin family protein	-
LAZ98_RS13075 (LAZ98_13075)	-	2591497..2592327 (-)	831	WP_017418143.1	STAS domain-containing protein	-
LAZ98_RS13085 (LAZ98_13085)	-	2592534..2592914 (-)	381	WP_224443286.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
LAZ98_RS13090 (LAZ98_13090)	-	2593151..2593396 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.81 Da Isoelectric Point: 9.3485

                                >NTDB_id=607836 LAZ98_RS13060 WP_014418378.1 2587799..2588869(-) (comGA) [Bacillus velezensis strain DMB07]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=607836 LAZ98_RS13060 WP_014418378.1 2587799..2588869(-) (comGA) [Bacillus velezensis strain DMB07]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC

ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0607836