Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   LAZ96_RS10125 Genome accession   NZ_CP083715
Coordinates   1960545..1961063 (-) Length   172 a.a.
NCBI ID   WP_004392979.1    Uniprot ID   A0A9Q3LJE1
Organism   Bacillus velezensis strain DMB05     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1943776..1961390 1960545..1961063 within 0


Gene organization within MGE regions


Location: 1943776..1961390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAZ96_RS10025 (LAZ96_10025) - 1944246..1945127 (+) 882 WP_025650105.1 DMT family transporter -
  LAZ96_RS10030 (LAZ96_10030) - 1945242..1945829 (+) 588 Protein_1975 HD domain-containing protein -
  LAZ96_RS10035 (LAZ96_10035) - 1945834..1946046 (-) 213 Protein_1976 PLP-dependent aminotransferase family protein -
  LAZ96_RS10040 (LAZ96_10040) - 1946454..1947287 (-) 834 WP_025650107.1 hypothetical protein -
  LAZ96_RS10045 (LAZ96_10045) - 1947432..1948007 (+) 576 WP_076983302.1 hypothetical protein -
  LAZ96_RS10050 (LAZ96_10050) - 1948018..1948455 (+) 438 WP_025650109.1 hypothetical protein -
  LAZ96_RS10055 (LAZ96_10055) - 1948558..1949364 (+) 807 WP_025650110.1 hypothetical protein -
  LAZ96_RS10060 (LAZ96_10060) - 1949351..1950163 (+) 813 WP_025650111.1 hypothetical protein -
  LAZ96_RS10065 (LAZ96_10065) - 1950178..1950849 (+) 672 WP_025650112.1 ATP-binding cassette domain-containing protein -
  LAZ96_RS10070 (LAZ96_10070) - 1950842..1951348 (+) 507 WP_032865466.1 hypothetical protein -
  LAZ96_RS21020 (LAZ96_10075) - 1951835..1952032 (+) 198 WP_224181425.1 restriction endonuclease -
  LAZ96_RS10080 (LAZ96_10080) - 1952201..1954270 (+) 2070 WP_071182101.1 thioredoxin domain-containing protein -
  LAZ96_RS10085 (LAZ96_10085) - 1954267..1955163 (-) 897 WP_017418443.1 CPBP family intramembrane glutamic endopeptidase -
  LAZ96_RS10090 (LAZ96_10090) - 1955378..1956709 (+) 1332 WP_014419380.1 MFS transporter -
  LAZ96_RS10095 (LAZ96_10095) - 1956743..1957123 (-) 381 WP_017418442.1 VOC family protein -
  LAZ96_RS10100 (LAZ96_10100) - 1957180..1958118 (-) 939 WP_025650114.1 LacI family DNA-binding transcriptional regulator -
  LAZ96_RS10105 (LAZ96_10105) xth 1958181..1958939 (-) 759 WP_025650115.1 exodeoxyribonuclease III -
  LAZ96_RS10110 (LAZ96_10110) - 1958936..1959493 (-) 558 WP_017418439.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LAZ96_RS10115 (LAZ96_10115) - 1959490..1960071 (-) 582 WP_017418438.1 bifunctional transcriptional activator/DNA repair enzyme AdaA -
  LAZ96_RS10120 (LAZ96_10120) rpsR 1960262..1960501 (-) 240 WP_004392983.1 30S ribosomal protein S18 -
  LAZ96_RS10125 (LAZ96_10125) ssbA 1960545..1961063 (-) 519 WP_004392979.1 single-stranded DNA-binding protein SsbA Machinery gene
  LAZ96_RS10130 (LAZ96_10130) rpsF 1961103..1961390 (-) 288 WP_004392977.1 30S ribosomal protein S6 -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 18745.31 Da        Isoelectric Point: 4.7621

>NTDB_id=607411 LAZ96_RS10125 WP_004392979.1 1960545..1961063(-) (ssbA) [Bacillus velezensis strain DMB05]
MLNRVVLVGRLTKDPELRYTPSGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNSGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=607411 LAZ96_RS10125 WP_004392979.1 1960545..1961063(-) (ssbA) [Bacillus velezensis strain DMB05]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAGCGGTGCGGCTGTCGC
CACGTTTACTCTTGCTGTGAATCGTACATTCACGAACCAGTCCGGAGAACGTGAAGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTTCTTAAAAAAGGAAGCCTTGCCGGCGTAGACGGCCGACTCCAGACAAGA
AATTATGAAAACCAGCAGGGACAGCGTGTCTTCGTGACAGAAGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAAAA
CAGCGGCGGTTCTGGATCAGGCGGATACAACGAAGGAAACAGCGGCGGAGGCCAATACTTTGGCGGAGGCCAAAATGATA
ATCCGTTCGGCGGAAATCAGAACAACCAGAGACGAAATCAAGGGAACAGCTTTAATGATGATCCATTTGCCAACGACGGC
AAGCCGATTGACATCTCGGATGATGATCTTCCGTTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

98.837

100

0.988
  ssb Latilactobacillus sakei subsp. sakei 23K

58.757

100

0.605
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395
  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378