Detailed information NTG0606234

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABEDC_RS07070
• Genome accession: NZ_CP083571
• Coordinates: 1431088..1432284 (+)
• Length: 398 a.a.
• NCBI ID: WP_004647070.1
• Uniprot ID: -
• Organism: Acinetobacter lwoffii strain ED9-5a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	1427850..1430129	1431088..1432284	flank	959

Gene organization within MGE regions

Location: 1427850..1432284

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABEDC_RS07055 (ABEDC_1471)	-	1427850..1429187 (-)	1338	WP_374952757.1	ISNCY family transposase	-
ABEDC_RS07060 (ABEDC_1472)	-	1429428..1430129 (+)	702	WP_004282345.1	IS1 family transposase	-
ABEDC_RS07065 (ABEDC_1473)	-	1430287..1430493 (+)	207	WP_374952758.1	3-hydroxybutyryl-CoA dehydrogenase	-
ABEDC_RS07070 (ABEDC_1474)	pilU	1431088..1432284 (+)	1197	WP_004647070.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 45141.72 Da
• Isoelectric Point: 6.6224

>NTDB_id=606234 ABEDC_RS07070 WP_004647070.1 1431088..1432284(+) (pilU) [Acinetobacter lwoffii strain ED9-5a]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLMSDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGEISYEEALRNADSTNELRLQIKLKSNRQDTTVATEATQFTMMQEPKAEEKQAENTSVSE

Nucleotide

• Length: 1197 bp

>NTDB_id=606234 ABEDC_RS07070 WP_004647070.1 1431088..1432284(+) (pilU) [Acinetobacter lwoffii strain ED9-5a]
ATGTTCACGGCAGAATTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACCAAGGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCGAGTATTAAACATCAAGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CAGCTGAAAAAACCAAGCTGTTTGCCTACAGCCTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTTGAATGT
AACTTTGCCATTACTGTACCTGGCGTCTCCCGTTTCCGGGTCAATGTGTTCCAGCAACAACTTAATGTCGGTATGGTAAT
TCGTACCATTACTGCAGAAATTCCAAATTTCCAGAAGCTGAAATTACCGGAATCTCTGAAACATGTGATTATGGAAAAAC
GTGGACTGGTATTGGTAGTCGGAGGAACAGGTTCCGGTAAATCAACTTCACTCGCAGCCATGATTGACTATCGAAATGAA
AATTCAGCCGGGCATATCATTACCGTAGAAGATCCGGTTGAATATGTGCACAAGCATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCCTGGCATAATGCGCTGAAAAATACCTTGCGTCAGGCACCAGATGTGATTCTGATCG
GCGAAATTCGCGATACTGAAACCATGGAACACGCGATTGCTTTTGCTGAAACTGGACATCTGTGTCTGGGAACGCTGCAC
TCGAACAATGCCAACCAGACTTTGGACCGGATTATCAATTTCTTCCCGGAAGAACGCCGGAACCAGCTATTAATGGATTT
GTCATCCAATATGAAGGCGATCATTTCACAGCGTCTGATTCGTACTCAAGATGGTAAAGGCCGTCGTGCAGCGATTGAAA
TCATGTTAAATACGCCACTGATGTCTGACCTGATTTTGAAAGGTGAATTCCACGAATTGAAAGCGATTATGGCGAAATCG
CGTGAACTGGGCATGCAGACCTTTGACCAGGCCTTATTTGACCTGTATAACCAGGGTGAAATTTCTTATGAAGAAGCCTT
GCGCAATGCCGATTCGACCAATGAACTGCGCTTACAAATCAAGCTGAAAAGTAACCGACAGGACACTACCGTAGCCACAG
AGGCAACCCAATTCACCATGATGCAAGAGCCAAAAGCGGAAGAGAAACAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.494	87.437	0.538
pilU	Acinetobacter baylyi ADP1	56.196	87.186	0.49
pilU	Vibrio cholerae strain A1552	52.394	89.196	0.467
pilT	Pseudomonas aeruginosa PAK	44.172	81.91	0.362