Detailed information NTG0603887

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K9C16_RS14900
• Genome accession: NZ_CP082952
• Coordinates: 3097850..3098968 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain Cl300
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3092850..3103968

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K9C16_RS14890 (K9C16_14825)	-	3095966..3096658 (-)	693	WP_032050220.1	YggS family pyridoxal phosphate-dependent enzyme	-
K9C16_RS14895 (K9C16_14830)	pilT	3096785..3097822 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
K9C16_RS14900 (K9C16_14835)	pilU	3097850..3098968 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K9C16_RS14905 (K9C16_14840)	fur	3099038..3099475 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
K9C16_RS14910 (K9C16_14845)	-	3099587..3099985 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
K9C16_RS14915 (K9C16_14850)	-	3100040..3100363 (-)	324	WP_000442589.1	RnfH family protein	-
K9C16_RS14920 (K9C16_14855)	-	3100363..3101430 (-)	1068	WP_031962994.1	membrane protein	-
K9C16_RS14925 (K9C16_14860)	-	3101583..3102038 (-)	456	WP_000782976.1	bacteriohemerythrin	-
K9C16_RS14930 (K9C16_14865)	-	3102291..3103139 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=603887 K9C16_RS14900 WP_000347036.1 3097850..3098968(+) (pilU) [Acinetobacter baumannii strain Cl300]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=603887 K9C16_RS14900 WP_000347036.1 3097850..3098968(+) (pilU) [Acinetobacter baumannii strain Cl300]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392