Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8U54_RS24490 Genome accession   NZ_CP082928
Coordinates   5292027..5293244 (-) Length   405 a.a.
NCBI ID   WP_249908218.1    Uniprot ID   -
Organism   Pseudomonas fulva strain ZJU1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5287027..5298244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8U54_RS24465 (K8U54_24415) - 5287095..5287865 (+) 771 WP_249908213.1 3-hydroxybutyrate dehydrogenase -
  K8U54_RS24470 (K8U54_24420) - 5287925..5289880 (+) 1956 WP_249908214.1 acetoacetate--CoA ligase -
  K8U54_RS24475 (K8U54_24425) - 5290026..5290742 (+) 717 WP_249908215.1 transporter substrate-binding domain-containing protein -
  K8U54_RS24480 (K8U54_24430) - 5290798..5291316 (+) 519 WP_249908216.1 thioredoxin family protein -
  K8U54_RS24485 (K8U54_24435) - 5291313..5291924 (-) 612 WP_249908217.1 DJ-1/PfpI family protein -
  K8U54_RS24490 (K8U54_24440) pilU 5292027..5293244 (-) 1218 WP_249908218.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8U54_RS24495 (K8U54_24445) - 5293388..5293666 (+) 279 WP_249908219.1 peptidylprolyl isomerase -
  K8U54_RS24500 (K8U54_24450) sseA 5293777..5294628 (+) 852 WP_249908220.1 3-mercaptopyruvate sulfurtransferase -
  K8U54_RS24505 (K8U54_24455) - 5294898..5295629 (-) 732 WP_249908221.1 ABC transporter ATP-binding protein -
  K8U54_RS24510 (K8U54_24460) - 5295629..5296450 (-) 822 WP_249908222.1 ABC transporter permease -
  K8U54_RS24515 (K8U54_24465) - 5296437..5297420 (-) 984 WP_249908223.1 aliphatic sulfonate ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44754.16 Da        Isoelectric Point: 5.7058

>NTDB_id=603676 K8U54_RS24490 WP_249908218.1 5292027..5293244(-) (pilU) [Pseudomonas fulva strain ZJU1]
MDLHAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSSEPLKAGAVAEIADGVMDVAQREEFERELEMNLAISLNGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTIRDLIKRNEFAEIKEIMEKSRNLGMQTFDQAL
IDLVNEGAIDEEEAVKNADSANNVRLKLKLYRDNPAVPAPAMQPAAAPAEVRPVAPGEPADWGLELKLEEIEEEAPPEDP
GRHGI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=603676 K8U54_RS24490 WP_249908218.1 5292027..5293244(-) (pilU) [Pseudomonas fulva strain ZJU1]
ATGGATCTCCATGCAATGCTGAAAATCCTCTCCAGCCAGGACGGCTCGGATCTCTACCTGTCGACCGGTGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTCAAGGCCCTGAGCAGCGAGCCGCTCAAGGCCGGCGCGGTGGCGGAAATCGCCGACGGGG
TGATGGATGTCGCCCAGCGCGAGGAATTCGAGCGCGAGCTGGAAATGAACCTGGCGATTTCCCTCAATGGCGTCGGGCGC
TTTCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTTGATATCCCGCGCTTCGA
GGACCTGAAACTGCCCGAAGTGCTGCTCAAGACGGTCATGGAGAAACGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCGACCTCGCTGGCCGCGCTGATCGACTACCGCAATCGCAACAGCGGCGGTCACATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGCGTGGATACGCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCCTGGCGTTCGCCGATACCGGGCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAAGACCGCCGCCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTCGTGTCCCAGCG
CCTGGTCAAGACCGTCGACGGCAAGCGCCGCGCGGCGGTCGAGGTGCTGCTCGGCACGCCGACCATCCGCGACCTGATCA
AGCGCAACGAGTTTGCCGAGATCAAGGAGATCATGGAAAAGTCCAGGAACCTCGGCATGCAGACTTTCGATCAGGCACTG
ATCGATCTGGTCAACGAAGGCGCCATCGACGAAGAAGAAGCGGTGAAGAACGCCGACTCGGCCAACAATGTGCGCCTCAA
GCTCAAACTCTATCGGGACAATCCCGCTGTTCCGGCGCCCGCAATGCAGCCAGCGGCTGCGCCAGCCGAGGTTCGCCCGG
TAGCGCCTGGCGAGCCTGCAGACTGGGGGCTGGAACTGAAGCTGGAAGAGATCGAGGAGGAGGCGCCACCCGAGGACCCG
GGGCGTCACGGTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.265

86.667

0.496
  pilU Acinetobacter baylyi ADP1

52.691

87.16

0.459
  pilU Vibrio cholerae strain A1552

51.558

87.16

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.73

91.358

0.363