Detailed information NTG0602660

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K8B71_RS02060
• Genome accession: NZ_CP082823
• Coordinates: 444332..445480 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 439332..450480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8B71_RS02035 (K8B71_02035)	-	440483..440779 (-)	297	WP_031627661.1	DUF167 domain-containing protein	-
K8B71_RS02040 (K8B71_02040)	-	440776..441369 (-)	594	WP_003084544.1	YggT family protein	-
K8B71_RS02045 (K8B71_02045)	proC	441380..442201 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
K8B71_RS02050 (K8B71_02050)	-	442213..442905 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
K8B71_RS02055 (K8B71_02055)	pilT	443120..444154 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
K8B71_RS02060 (K8B71_02060)	pilU	444332..445480 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
K8B71_RS02065 (K8B71_02065)	czcD	445488..446387 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
K8B71_RS02070 (K8B71_02070)	-	446541..446945 (+)	405	WP_003084560.1	NINE protein	-
K8B71_RS02075 (K8B71_02075)	-	447188..448561 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
K8B71_RS02080 (K8B71_02080)	-	448558..449742 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=602660 K8B71_RS02060 WP_003110016.1 444332..445480(+) (pilU) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=602660 K8B71_RS02060 WP_003110016.1 444332..445480(+) (pilU) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCTGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382