Detailed information NTG0602625

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K8B69_RS02235
• Genome accession: NZ_CP082822
• Coordinates: 462460..463608 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 457460..468608

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8B69_RS02210 (K8B69_02210)	-	458611..458907 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
K8B69_RS02215 (K8B69_02215)	-	458904..459497 (-)	594	WP_003084544.1	YggT family protein	-
K8B69_RS02220 (K8B69_02220)	proC	459508..460329 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
K8B69_RS02225 (K8B69_02225)	-	460341..461033 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
K8B69_RS02230 (K8B69_02230)	pilT	461248..462282 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
K8B69_RS02235 (K8B69_02235)	pilU	462460..463608 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
K8B69_RS02240 (K8B69_02240)	czcD	463616..464515 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
K8B69_RS02245 (K8B69_02245)	-	464669..465073 (+)	405	WP_003084560.1	NINE protein	-
K8B69_RS02250 (K8B69_02250)	-	465316..466689 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
K8B69_RS02255 (K8B69_02255)	-	466686..467870 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=602625 K8B69_RS02235 WP_003100959.1 462460..463608(+) (pilU) [Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=602625 K8B69_RS02235 WP_003100959.1 462460..463608(+) (pilU) [Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382