Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K6754_RS01635 Genome accession   NZ_CP082317
Coordinates   348280..348873 (+) Length   197 a.a.
NCBI ID   WP_064354357.1    Uniprot ID   A0AAW7A2J1
Organism   Vibrio alginolyticus strain SXV3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343280..353873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6754_RS01620 (K6754_01620) - 344006..346567 (-) 2562 WP_025768081.1 penicillin-binding protein 1A -
  K6754_RS01625 (K6754_01625) pilM 346712..347728 (+) 1017 WP_213912483.1 type IV pilus biogenesis protein PilM Machinery gene
  K6754_RS01630 (K6754_01630) pilN 347712..348287 (+) 576 WP_213912482.1 PilN domain-containing protein Machinery gene
  K6754_RS01635 (K6754_01635) pilO 348280..348873 (+) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6754_RS01640 (K6754_01640) pilP 348863..349378 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6754_RS01645 (K6754_01645) pilQ 349404..351149 (+) 1746 WP_238974988.1 type IV pilus secretin PilQ Machinery gene
  K6754_RS01650 (K6754_01650) aroK 351337..351855 (+) 519 WP_005381319.1 shikimate kinase AroK -
  K6754_RS01655 (K6754_01655) aroB 351888..352988 (+) 1101 WP_005382764.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0885

>NTDB_id=601362 K6754_RS01635 WP_064354357.1 348280..348873(+) (pilO) [Vibrio alginolyticus strain SXV3]
MVDWQDLEIDEMAEWPLLPQIIVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=601362 K6754_RS01635 WP_064354357.1 348280..348873(+) (pilO) [Vibrio alginolyticus strain SXV3]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGATACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

86.842

96.447

0.838
  pilO Vibrio cholerae strain A1552

70.157

96.954

0.68