Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K6754_RS01625 Genome accession   NZ_CP082317
Coordinates   346712..347728 (+) Length   338 a.a.
NCBI ID   WP_213912483.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain SXV3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341712..352728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6754_RS01610 (K6754_01610) - 342143..342871 (-) 729 WP_005382781.1 redoxin family protein -
  K6754_RS01615 (K6754_01615) oxyR 343041..343952 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  K6754_RS01620 (K6754_01620) - 344006..346567 (-) 2562 WP_025768081.1 penicillin-binding protein 1A -
  K6754_RS01625 (K6754_01625) pilM 346712..347728 (+) 1017 WP_213912483.1 type IV pilus biogenesis protein PilM Machinery gene
  K6754_RS01630 (K6754_01630) pilN 347712..348287 (+) 576 WP_213912482.1 PilN domain-containing protein Machinery gene
  K6754_RS01635 (K6754_01635) pilO 348280..348873 (+) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6754_RS01640 (K6754_01640) pilP 348863..349378 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6754_RS01645 (K6754_01645) pilQ 349404..351149 (+) 1746 WP_238974988.1 type IV pilus secretin PilQ Machinery gene
  K6754_RS01650 (K6754_01650) aroK 351337..351855 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37610.92 Da        Isoelectric Point: 6.7742

>NTDB_id=601360 K6754_RS01625 WP_213912483.1 346712..347728(+) (pilM) [Vibrio alginolyticus strain SXV3]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYTLLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGARNVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLLAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=601360 K6754_RS01625 WP_213912483.1 346712..347728(+) (pilM) [Vibrio alginolyticus strain SXV3]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACACTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAGAAATGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCTCGCGGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTTGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCATAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTGGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.149

99.112

0.497