Detailed information NTG0601308

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NE424_RS08445
• Genome accession: NZ_CP098795
• Coordinates: 1757695..1758813 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain VB280821
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1752695..1763813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NE424_RS08435 (NE424_08435)	-	1755811..1756503 (-)	693	WP_031965550.1	YggS family pyridoxal phosphate-dependent enzyme	-
NE424_RS08440 (NE424_08440)	pilT	1756630..1757667 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
NE424_RS08445 (NE424_08445)	pilU	1757695..1758813 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NE424_RS08450 (NE424_08450)	fur	1758882..1759319 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
NE424_RS08455 (NE424_08455)	bamE	1759431..1759829 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
NE424_RS08460 (NE424_08460)	-	1759884..1760207 (-)	324	WP_000442585.1	RnfH family protein	-
NE424_RS08465 (NE424_08465)	-	1760207..1761274 (-)	1068	WP_002084046.1	hypothetical protein	-
NE424_RS08470 (NE424_08470)	-	1761427..1761882 (-)	456	WP_000782976.1	bacteriohemerythrin	-
NE424_RS08475 (NE424_08475)	-	1762135..1762983 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=601308 NE424_RS08445 WP_000347036.1 1757695..1758813(+) (pilU) [Acinetobacter baumannii strain VB280821]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=601308 NE424_RS08445 WP_000347036.1 1757695..1758813(+) (pilU) [Acinetobacter baumannii strain VB280821]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCAGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTTGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392