Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K6750_RS01565 Genome accession   NZ_CP082313
Coordinates   333785..334378 (+) Length   197 a.a.
NCBI ID   WP_042523672.1    Uniprot ID   A0A7Y0R197
Organism   Vibrio alginolyticus strain ZZV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328785..339378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6750_RS01550 (K6750_01550) - 329511..332072 (-) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -
  K6750_RS01555 (K6750_01555) pilM 332217..333233 (+) 1017 WP_005382774.1 type IV pilus assembly protein PilM Machinery gene
  K6750_RS01560 (K6750_01560) pilN 333217..333792 (+) 576 WP_064368815.1 PilN domain-containing protein Machinery gene
  K6750_RS01565 (K6750_01565) pilO 333785..334378 (+) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6750_RS01570 (K6750_01570) pilP 334368..334883 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6750_RS01575 (K6750_01575) pilQ 334909..336654 (+) 1746 WP_042523674.1 type IV pilus secretin PilQ family protein Machinery gene
  K6750_RS01580 (K6750_01580) aroK 336842..337360 (+) 519 WP_005381319.1 shikimate kinase AroK -
  K6750_RS01585 (K6750_01585) aroB 337393..338493 (+) 1101 WP_238966806.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22999.53 Da        Isoelectric Point: 5.0948

>NTDB_id=601268 K6750_RS01565 WP_042523672.1 333785..334378(+) (pilO) [Vibrio alginolyticus strain ZZV2]
MVDWQDLEIDEMAEWPLLPQIVVIVLLIMAIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEAKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=601268 K6750_RS01565 WP_042523672.1 333785..334378(+) (pilO) [Vibrio alginolyticus strain ZZV2]
GTGGTTGATTGGCAAGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGTACTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATTCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGTGCTAATACCTATCA
GTTTAAGCCGGAGGAGGCGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0R197

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685