Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K6750_RS01555 Genome accession   NZ_CP082313
Coordinates   332217..333233 (+) Length   338 a.a.
NCBI ID   WP_005382774.1    Uniprot ID   A0AAP5Z0M3
Organism   Vibrio alginolyticus strain ZZV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327217..338233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6750_RS01540 (K6750_01540) - 327649..328377 (-) 729 WP_005382781.1 redoxin family protein -
  K6750_RS01545 (K6750_01545) oxyR 328547..329458 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  K6750_RS01550 (K6750_01550) - 329511..332072 (-) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -
  K6750_RS01555 (K6750_01555) pilM 332217..333233 (+) 1017 WP_005382774.1 type IV pilus assembly protein PilM Machinery gene
  K6750_RS01560 (K6750_01560) pilN 333217..333792 (+) 576 WP_064368815.1 PilN domain-containing protein Machinery gene
  K6750_RS01565 (K6750_01565) pilO 333785..334378 (+) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6750_RS01570 (K6750_01570) pilP 334368..334883 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6750_RS01575 (K6750_01575) pilQ 334909..336654 (+) 1746 WP_042523674.1 type IV pilus secretin PilQ family protein Machinery gene
  K6750_RS01580 (K6750_01580) aroK 336842..337360 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37609.97 Da        Isoelectric Point: 7.2224

>NTDB_id=601266 K6750_RS01555 WP_005382774.1 332217..333233(+) (pilM) [Vibrio alginolyticus strain ZZV2]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=601266 K6750_RS01555 WP_005382774.1 332217..333233(+) (pilM) [Vibrio alginolyticus strain ZZV2]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACTATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503