Detailed information NTG0600505

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K6751_RS10000
• Genome accession: NZ_CP082244
• Coordinates: 2149739..2150953 (+)
• Length: 404 a.a.
• NCBI ID: WP_222901567.1
• Uniprot ID: -
• Organism: Metapseudomonas otitidis strain CSMC7.1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2144739..2155953

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6751_RS09975 (K6751_09975)	-	2144877..2145266 (-)	390	WP_222901562.1	GtrA family protein	-
K6751_RS09980 (K6751_09980)	-	2145263..2146264 (-)	1002	WP_222901563.1	glycosyltransferase family 2 protein	-
K6751_RS09985 (K6751_09985)	-	2146257..2147750 (-)	1494	WP_222901564.1	ArnT family glycosyltransferase	-
K6751_RS28775 (K6751_09990)	-	2147894..2149198 (-)	1305	WP_222901565.1	methyl-accepting chemotaxis protein	-
K6751_RS09995 (K6751_09995)	-	2149317..2149595 (-)	279	WP_222901566.1	peptidylprolyl isomerase	-
K6751_RS10000 (K6751_10000)	pilU	2149739..2150953 (+)	1215	WP_222901567.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K6751_RS10005 (K6751_10005)	-	2150982..2151716 (-)	735	WP_233473466.1	substrate-binding periplasmic protein	-
K6751_RS10010 (K6751_10010)	-	2151802..2152527 (-)	726	WP_222901569.1	alpha/beta fold hydrolase	-
K6751_RS10015 (K6751_10015)	-	2152508..2153299 (-)	792	WP_044409054.1	crotonase/enoyl-CoA hydratase family protein	-
K6751_RS10020 (K6751_10020)	-	2153652..2155328 (+)	1677	WP_044409057.1	DEAD/DEAH box helicase	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44490.89 Da
• Isoelectric Point: 6.6002

>NTDB_id=600505 K6751_RS10000 WP_222901567.1 2149739..2150953(+) (pilU) [Metapseudomonas otitidis strain CSMC7.1]
MDLPSMLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLSADPLKPGDVARIAESIMDPEQRVEFNHELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQAL
FDLVVEGAISEDEAIKNADSANNLRLKLKLYRDGPTPAPTPAPAAAPTPPPAATRTQDAASWGLELKLEELPEETPPEDP
GKHF

Nucleotide

• Length: 1215 bp

>NTDB_id=600505 K6751_RS10000 WP_222901567.1 2149739..2150953(+) (pilU) [Metapseudomonas otitidis strain CSMC7.1]
ATGGATCTCCCTTCGATGCTGAAGGTCCTTGCCTCCCAGGATGGCTCGGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGCTGAGCGCCGACCCGCTCAAGCCGGGGGATGTCGCGCGTATCGCCGAGTCGA
TCATGGACCCGGAGCAACGCGTCGAATTCAACCATGAGCTGGAGATGAACCTGGCGATCTCGGTGCCCAACGTGGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTCTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCCCGCTTCGA
GGACCTCAAGCTGCCCGAGGTACTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
GGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATTGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGTCACCTGGCCATTTCCACCCTGCATGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAACGCCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACTTCCGACGGCAAGCGTCGCGCGGCGGTGGAGGTGCTGCTGGGCACTTCCACCATCAGCGACCTGATCA
AGCGCGGTGACTTCAGCTCCATCAAGGAGATCATGGAGAAGTCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTGGTGGAAGGCGCCATCAGCGAGGACGAGGCGATCAAGAACGCCGACTCCGCCAACAACCTGCGCCTCAA
GCTGAAGCTCTACCGCGACGGCCCGACTCCCGCGCCGACGCCCGCTCCCGCCGCCGCACCCACCCCGCCACCGGCCGCGA
CCCGTACCCAGGACGCAGCCAGTTGGGGGCTGGAGCTGAAGCTGGAGGAACTGCCCGAGGAAACGCCGCCGGAAGATCCG
GGCAAGCATTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.881	0.488
pilU	Acinetobacter baylyi ADP1	52.925	88.861	0.47
pilU	Vibrio cholerae strain A1552	52.125	87.376	0.455
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.73	91.584	0.364