Detailed information NTG0600493

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K6751_RS01910
• Genome accession: NZ_CP082244
• Coordinates: 381293..382438 (+)
• Length: 381 a.a.
• NCBI ID: WP_044403589.1
• Uniprot ID: A0AB36F620
• Organism: Metapseudomonas otitidis strain CSMC7.1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 376293..387438

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6751_RS01880 (K6751_01880)	-	376382..376678 (-)	297	WP_044403598.1	DUF167 domain-containing protein	-
K6751_RS01885 (K6751_01885)	-	376678..377271 (-)	594	WP_074967194.1	YggT family protein	-
K6751_RS01890 (K6751_01890)	-	377363..378550 (-)	1188	WP_222900744.1	hypothetical protein	-
K6751_RS01895 (K6751_01895)	proC	378553..379371 (-)	819	WP_222900745.1	pyrroline-5-carboxylate reductase	-
K6751_RS01900 (K6751_01900)	-	379389..380081 (-)	693	WP_160480527.1	YggS family pyridoxal phosphate-dependent enzyme	-
K6751_RS01905 (K6751_01905)	pilT	380150..381184 (+)	1035	WP_142012818.1	type IV pilus twitching motility protein PilT	Machinery gene
K6751_RS01910 (K6751_01910)	pilU	381293..382438 (+)	1146	WP_044403589.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K6751_RS28750 (K6751_01915)	-	382380..383825 (-)	1446	WP_222900746.1	TIGR03915 family putative DNA repair protein	-
K6751_RS01920 (K6751_01920)	-	383825..385036 (-)	1212	WP_069566424.1	putative DNA modification/repair radical SAM protein	-
K6751_RS01925 (K6751_01925)	-	385143..385538 (+)	396	WP_074967204.1	NINE protein	-
K6751_RS01930 (K6751_01930)	-	385597..386868 (-)	1272	WP_172432364.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42485.80 Da
• Isoelectric Point: 6.7727

>NTDB_id=600493 K6751_RS01910 WP_044403589.1 381293..382438(+) (pilU) [Metapseudomonas otitidis strain CSMC7.1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVMPVTKNPMSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEQLKLPEVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLVADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSISGGLSLEVSEDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=600493 K6751_RS01910 WP_044403589.1 381293..382438(+) (pilU) [Metapseudomonas otitidis strain CSMC7.1]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGTTCGGACCTGTTCATCACGGCCGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGGTCATGCCGGTCACCAAGAACCCCATGTCGCCGGAGCAGACCCGGGAAACCGTGCTGGGGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATTAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGCGCTTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
GCAGCTCAAGCTGCCCGAGGTGCTGAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGCTCTTCGTCGGTGCCACCGGCA
CCGGCAAGTCCACGTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCTCCGGCCACATCATCTCCATCGAAGAC
CCCATCGAATACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGTGAAGTGGGGATCGACACCGAGTCCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGGACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCTGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGTGGCCGACCTGA
TCCGCAAGGGCGAGGTTCACGAGCTCAAGCCGCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCCCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAGACCGACGGCGAACACCTGACCAGCATCAGTGGCGGGCTGTCCCTGGAAGTCTCGGAAG
ACGATCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.126	100	0.921
pilU	Acinetobacter baylyi ADP1	63.866	93.701	0.598
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386