NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	NBY12_RS20985	Genome accession	NZ_CP098223
Coordinates	4360372..4361610 (-)	Length	412 a.a.
NCBI ID	WP_072001002.1	Uniprot ID	-
Organism	Escherichia coli strain Z0117EC0005
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4355372..4366610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NBY12_RS20965 (NBY12_20990)	dam	4355984..4356820 (-)	837	WP_000742143.1	adenine-specific DNA-methyltransferase	-
NBY12_RS20970 (NBY12_20995)	damX	4356927..4358213 (-)	1287	WP_032303912.1	cell division protein DamX	-
NBY12_RS20975 (NBY12_21000)	aroB	4358305..4359393 (-)	1089	WP_000439850.1	3-dehydroquinate synthase	-
NBY12_RS20980 (NBY12_21005)	aroK	4359450..4359971 (-)	522	WP_000818618.1	shikimate kinase AroK	-
NBY12_RS20985 (NBY12_21010)	comE	4360372..4361610 (-)	1239	WP_072001002.1	DNA uptake porin HofQ	Machinery gene
NBY12_RS20990 (NBY12_21015)	hofP	4361522..4361926 (-)	405	WP_001264139.1	DNA utilization protein HofP	-
NBY12_RS20995 (NBY12_21020)	hofO	4361916..4362356 (-)	441	WP_032303915.1	DNA utilization protein HofO	-
NBY12_RS21000 (NBY12_21025)	hofN	4362340..4362879 (-)	540	WP_039020609.1	DNA utilization protein HofN	-
NBY12_RS21005 (NBY12_21030)	hofM	4362879..4363658 (-)	780	WP_001354999.1	DNA utilization protein HofM	-
NBY12_RS21010 (NBY12_21035)	mrcA	4363778..4366330 (+)	2553	WP_032303918.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44722.24 Da Isoelectric Point: 5.9488

                                >NTDB_id=598540 NBY12_RS20985 WP_072001002.1 4360372..4361610(-) (comE) [Escherichia coli strain Z0117EC0005]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQVLQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQTGGVGQVTTLGSDLSVATATTHVGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=598540 NBY12_RS20985 WP_072001002.1 4360372..4361610(-) (comE) [Escherichia coli strain Z0117EC0005]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGTACTACAAACTGTAGTGAAAAGCGCCGGACTG

ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA

GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAGCGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAACTGGTGGCGTTG

GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGCCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGTGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae 86-028NP	37.3	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	38.015	100	0.381
pilQ	Vibrio cholerae strain A1552	38.015	100	0.381
comE	Glaesserella parasuis strain SC1401	36.105	100	0.369

Detailed information NTG0598540