Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NBY12_RS18980 Genome accession   NZ_CP098223
Coordinates   3971655..3973148 (-) Length   497 a.a.
NCBI ID   WP_032303779.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966655..3978148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY12_RS18950 (NBY12_18970) gspK 3967449..3968426 (-) 978 WP_032303774.1 type II secretion system minor pseudopilin GspK -
  NBY12_RS18955 (NBY12_18975) gspJ 3968423..3969028 (-) 606 WP_032303775.1 type II secretion system minor pseudopilin GspJ -
  NBY12_RS18960 (NBY12_18980) gspI 3969025..3969396 (-) 372 WP_032303776.1 type II secretion system minor pseudopilin GspI -
  NBY12_RS18965 (NBY12_18985) gspH 3969393..3969956 (-) 564 WP_032303777.1 type II secretion system minor pseudopilin GspH -
  NBY12_RS18970 (NBY12_18990) gspG 3969960..3970415 (-) 456 WP_001087291.1 type II secretion system major pseudopilin GspG -
  NBY12_RS18975 (NBY12_18995) gspF 3970432..3971655 (-) 1224 WP_032303778.1 type II secretion system inner membrane protein GspF -
  NBY12_RS18980 (NBY12_19000) pilB/pilB1 3971655..3973148 (-) 1494 WP_032303779.1 type II secretion system ATPase GspE Machinery gene
  NBY12_RS18985 (NBY12_19005) gspD 3973148..3975208 (-) 2061 WP_032303780.1 type II secretion system secretin GspD -
  NBY12_RS18990 (NBY12_19010) gspC 3975238..3976191 (-) 954 WP_000135097.1 type II secretion system protein GspC -
  NBY12_RS18995 (NBY12_19015) gspS2 3976207..3976617 (-) 411 WP_001332998.1 type II secretion system pilot lipoprotein GspS-beta -
  NBY12_RS19000 (NBY12_19020) pilD 3976683..3977492 (-) 810 WP_001332999.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55159.23 Da        Isoelectric Point: 5.9800

>NTDB_id=598531 NBY12_RS18980 WP_032303779.1 3971655..3973148(-) (pilB/pilB1) [Escherichia coli strain Z0117EC0005]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=598531 NBY12_RS18980 WP_032303779.1 3971655..3973148(-) (pilB/pilB1) [Escherichia coli strain Z0117EC0005]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGCCATGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAATCACTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCGTCATTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGCCTGACGGGCGTGATGGCGCAGCGACTTGTTCGCACACTGTGCACCGACTGCCGCCAGCCCGCGCCTGCC
ACTGATGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTTGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTAACGATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

47.118

80.282

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362