Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   J450_RS03440 Genome accession   NC_021738
Coordinates   738296..739609 (+) Length   437 a.a.
NCBI ID   WP_006252205.1    Uniprot ID   A0A378NH42
Organism   Mannheimia haemolytica D171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733296..744609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J450_RS03415 (J450_03325) - 733450..736014 (-) 2565 WP_020824178.1 penicillin-binding protein 1A -
  J450_RS03420 (J450_03330) - 736138..736836 (+) 699 WP_006247933.1 hypothetical protein -
  J450_RS03425 (J450_03335) - 736812..737333 (+) 522 WP_006247934.1 hypothetical protein -
  J450_RS03430 (J450_03340) - 737335..737871 (+) 537 WP_006247935.1 hypothetical protein -
  J450_RS03435 (J450_03345) - 737874..738287 (+) 414 WP_006247936.1 hypothetical protein -
  J450_RS03440 (J450_03350) comE 738296..739609 (+) 1314 WP_006252205.1 type IV pilus secretin PilQ Machinery gene
  J450_RS03445 (J450_03355) nusB 739690..740103 (+) 414 WP_006247938.1 transcription antitermination factor NusB -
  J450_RS03450 (J450_03360) thiL 740166..741146 (+) 981 WP_006247939.1 thiamine-phosphate kinase -
  J450_RS03455 (J450_03365) - 741151..741624 (+) 474 WP_006247940.1 phosphatidylglycerophosphatase A -
  J450_RS03460 (J450_03370) - 741631..742254 (+) 624 WP_006247941.1 LysE family transporter -
  J450_RS03465 (J450_03375) ruvA 742366..742971 (+) 606 WP_006252204.1 Holliday junction branch migration protein RuvA -
  J450_RS03470 (J450_03380) ruvB 743108..744112 (+) 1005 WP_006252203.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48468.47 Da        Isoelectric Point: 7.5799

>NTDB_id=59793 J450_RS03440 WP_006252205.1 738296..739609(+) (comE) [Mannheimia haemolytica D171]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=59793 J450_RS03440 WP_006252205.1 738296..739609(+) (comE) [Mannheimia haemolytica D171]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCACAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378NH42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.073

99.771

0.629
  comE Haemophilus influenzae Rd KW20

51.034

99.542

0.508
  comE Haemophilus influenzae 86-028NP

50.345

99.542

0.501
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.192

96.339

0.378
  pilQ Vibrio cholerae strain A1552

39.192

96.339

0.378
  pilQ Vibrio campbellii strain DS40M4

37.355

98.627

0.368

Multiple sequence alignment