NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	K4L72_RS13170	Genome accession	NZ_CP081304
Coordinates	2696151..2696765 (-)	Length	204 a.a.
NCBI ID	WP_015387983.1	Uniprot ID	A0A6A8LHQ3
Organism	Bacillus velezensis strain CL-4
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2691151..2701765

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K4L72_RS13140 (K4L72_13140)	rpsT	2691451..2691717 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
K4L72_RS13145 (K4L72_13145)	holA	2691734..2692775 (-)	1042	Protein_2572	DNA polymerase III subunit delta	-
K4L72_RS13150 (K4L72_13150)	-	2692815..2692969 (-)	155	Protein_2573	hypothetical protein	-
K4L72_RS13155 (K4L72_13155)	-	2693010..2693144 (+)	135	WP_003152870.1	YqzM family protein	-
K4L72_RS13160 (K4L72_13160)	comEC	2693163..2695514 (-)	2352	WP_063174744.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K4L72_RS13165 (K4L72_13165)	-	2695515..2696084 (-)	570	WP_003152868.1	ComE operon protein 2	-
K4L72_RS13170 (K4L72_13170)	comEA	2696151..2696765 (-)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
K4L72_RS13175 (K4L72_13175)	comER	2696824..2697645 (+)	822	WP_014305449.1	late competence protein ComER	-
K4L72_RS13180 (K4L72_13180)	-	2697714..2698451 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
K4L72_RS13185 (K4L72_13185)	rsfS	2698448..2698804 (-)	357	WP_003152864.1	ribosome silencing factor	-
K4L72_RS13190 (K4L72_13190)	yqeK	2698801..2699382 (-)	582	WP_063174743.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K4L72_RS13195 (K4L72_13195)	-	2699372..2699941 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
K4L72_RS13200 (K4L72_13200)	yhbY	2699952..2700242 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
K4L72_RS13205 (K4L72_13205)	aroE	2700236..2701078 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22255.41 Da Isoelectric Point: 5.5211

                                >NTDB_id=595915 K4L72_RS13170 WP_015387983.1 2696151..2696765(-) (comEA) [Bacillus velezensis strain CL-4]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=595915 K4L72_RS13170 WP_015387983.1 2696151..2696765(-) (comEA) [Bacillus velezensis strain CL-4]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0595915