Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   K4L72_RS13160 Genome accession   NZ_CP081304
Coordinates   2693163..2695514 (-) Length   783 a.a.
NCBI ID   WP_063174744.1    Uniprot ID   -
Organism   Bacillus velezensis strain CL-4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2688163..2700514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4L72_RS13125 (K4L72_13125) - 2688526..2688864 (-) 339 WP_003152882.1 YqxA family protein -
  K4L72_RS13130 (K4L72_13130) spoIIP 2688881..2690074 (-) 1194 WP_063174745.1 stage II sporulation protein P -
  K4L72_RS13135 (K4L72_13135) gpr 2690142..2691248 (-) 1107 WP_003152878.1 GPR endopeptidase -
  K4L72_RS13140 (K4L72_13140) rpsT 2691451..2691717 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  K4L72_RS13145 (K4L72_13145) holA 2691734..2692775 (-) 1042 Protein_2572 DNA polymerase III subunit delta -
  K4L72_RS13150 (K4L72_13150) - 2692815..2692969 (-) 155 Protein_2573 hypothetical protein -
  K4L72_RS13155 (K4L72_13155) - 2693010..2693144 (+) 135 WP_003152870.1 YqzM family protein -
  K4L72_RS13160 (K4L72_13160) comEC 2693163..2695514 (-) 2352 WP_063174744.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  K4L72_RS13165 (K4L72_13165) - 2695515..2696084 (-) 570 WP_003152868.1 ComE operon protein 2 -
  K4L72_RS13170 (K4L72_13170) comEA 2696151..2696765 (-) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  K4L72_RS13175 (K4L72_13175) comER 2696824..2697645 (+) 822 WP_014305449.1 late competence protein ComER -
  K4L72_RS13180 (K4L72_13180) - 2697714..2698451 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  K4L72_RS13185 (K4L72_13185) rsfS 2698448..2698804 (-) 357 WP_003152864.1 ribosome silencing factor -
  K4L72_RS13190 (K4L72_13190) yqeK 2698801..2699382 (-) 582 WP_063174743.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  K4L72_RS13195 (K4L72_13195) - 2699372..2699941 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  K4L72_RS13200 (K4L72_13200) yhbY 2699952..2700242 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86735.75 Da        Isoelectric Point: 9.1033

>NTDB_id=595913 K4L72_RS13160 WP_063174744.1 2693163..2695514(-) (comEC) [Bacillus velezensis strain CL-4]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVIICLVSFCVYFFLYTVCDAVNVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRRIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLASFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFNMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFINAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=595913 K4L72_RS13160 WP_063174744.1 2693163..2695514(-) (comEC) [Bacillus velezensis strain CL-4]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGGATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTATCATCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGTGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAAGAAAAGAGACGCATTGAACAGCTTGAACCGGGGATGCGCTGCACATTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATAACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATATTGCTGCTGCT
GTTTTTACCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGCCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTAATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAACGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Competence protein (Competence)

(208-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.606

98.595

0.558