Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   M8Z96_RS26680 Genome accession   NZ_CP097556
Coordinates   5754571..5755194 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain B2.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5749571..5760194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8Z96_RS26660 (M8Z96_26630) aroB 5750166..5751272 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  M8Z96_RS26665 (M8Z96_26635) aroK 5751322..5751840 (-) 519 WP_034076693.1 shikimate kinase AroK -
  M8Z96_RS26670 (M8Z96_26640) pilQ 5751852..5753996 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  M8Z96_RS26675 (M8Z96_26645) comL/pilP 5754050..5754574 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  M8Z96_RS26680 (M8Z96_26650) pilO 5754571..5755194 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  M8Z96_RS26685 (M8Z96_26655) pilN 5755191..5755787 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  M8Z96_RS26690 (M8Z96_26660) pilM 5755787..5756851 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  M8Z96_RS26695 (M8Z96_26665) - 5757036..5759507 (+) 2472 WP_043089239.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=595256 M8Z96_RS26680 WP_003103268.1 5754571..5755194(-) (pilO) [Pseudomonas aeruginosa strain B2.1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=595256 M8Z96_RS26680 WP_003103268.1 5754571..5755194(-) (pilO) [Pseudomonas aeruginosa strain B2.1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401