Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K1516_RS04850 Genome accession   NZ_CP080573
Coordinates   1050842..1052005 (+) Length   387 a.a.
NCBI ID   WP_049400675.1    Uniprot ID   A0AA42HYU0
Organism   Stenotrophomonas maltophilia strain FZD2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045842..1057005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1516_RS04820 (K1516_04830) soxR 1045974..1046408 (+) 435 WP_094002028.1 redox-sensitive transcriptional activator SoxR -
  K1516_RS04825 (K1516_04835) - 1046482..1047084 (-) 603 WP_094002029.1 short chain dehydrogenase -
  K1516_RS04830 (K1516_04840) - 1047185..1048060 (+) 876 WP_248859466.1 LysR family transcriptional regulator -
  K1516_RS04835 (K1516_04845) proC 1048121..1048942 (-) 822 WP_248859475.1 pyrroline-5-carboxylate reductase -
  K1516_RS04840 (K1516_04850) - 1048961..1049638 (-) 678 WP_197611339.1 YggS family pyridoxal phosphate-dependent enzyme -
  K1516_RS04845 (K1516_04855) pilT 1049726..1050763 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  K1516_RS04850 (K1516_04860) pilU 1050842..1052005 (+) 1164 WP_049400675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K1516_RS04855 (K1516_04865) - 1052128..1052769 (+) 642 WP_164161046.1 hypothetical protein -
  K1516_RS04860 (K1516_04870) - 1052881..1053864 (+) 984 WP_153856475.1 aldo/keto reductase -
  K1516_RS04865 (K1516_04875) - 1053931..1054830 (-) 900 WP_164161050.1 LysR family transcriptional regulator -
  K1516_RS04870 (K1516_04880) - 1055133..1055345 (+) 213 WP_180846484.1 DUF4287 domain-containing protein -
  K1516_RS04875 (K1516_04885) - 1055441..1055890 (+) 450 WP_180846483.1 GNAT family N-acetyltransferase -
  K1516_RS04880 (K1516_04890) - 1055984..1056691 (+) 708 WP_088028269.1 YitT family protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43172.60 Da        Isoelectric Point: 6.6615

>NTDB_id=594150 K1516_RS04850 WP_049400675.1 1050842..1052005(+) (pilU) [Stenotrophomonas maltophilia strain FZD2]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=594150 K1516_RS04850 WP_049400675.1 1050842..1052005(+) (pilU) [Stenotrophomonas maltophilia strain FZD2]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCCGACCTGTTCATCACCGCAGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAGTCGACCTCGCTGGCAGCGATGATCGGCTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAAGTCGGCATCGATACCGACAGTTGGGAAGCGGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGACCTGTCGCTGAACCTCAAGGGCGTGGTTGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGTTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGA
GGACGCGCTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

94.832

0.643
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395