Detailed information NTG0593525

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KMW38_RS02010
• Genome accession: NZ_CP080518
• Coordinates: 426643..427791 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain YY322
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 421643..432791

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KMW38_RS01985 (KMW38_01985)	-	422794..423090 (-)	297	WP_031627661.1	DUF167 domain-containing protein	-
KMW38_RS01990 (KMW38_01990)	-	423087..423680 (-)	594	WP_003084544.1	YggT family protein	-
KMW38_RS01995 (KMW38_01995)	proC	423691..424512 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
KMW38_RS02000 (KMW38_02000)	-	424524..425216 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KMW38_RS02005 (KMW38_02005)	pilT	425431..426465 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KMW38_RS02010 (KMW38_02010)	pilU	426643..427791 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KMW38_RS02015 (KMW38_02015)	czcD	427799..428698 (-)	900	WP_003162016.1	CDF family zinc transporter CzcD	-
KMW38_RS02020 (KMW38_02020)	-	428852..429256 (+)	405	WP_003084560.1	NINE protein	-
KMW38_RS02025 (KMW38_02025)	-	429499..430872 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
KMW38_RS02030 (KMW38_02030)	-	430869..432053 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=593525 KMW38_RS02010 WP_003100959.1 426643..427791(+) (pilU) [Pseudomonas aeruginosa strain YY322]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=593525 KMW38_RS02010 WP_003100959.1 426643..427791(+) (pilU) [Pseudomonas aeruginosa strain YY322]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382