Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LDH51_RS05375 Genome accession   NZ_CP097001
Coordinates   1079384..1080877 (+) Length   497 a.a.
NCBI ID   WP_000249348.1    Uniprot ID   B7LGA7
Organism   Escherichia coli strain NCCP 15734     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1074384..1085877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDH51_RS05355 pilD 1075032..1075841 (+) 810 WP_001297673.1 prepilin peptidase PppA Machinery gene
  LDH51_RS05360 gspS2 1075907..1076317 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  LDH51_RS05365 gspC 1076335..1077294 (+) 960 WP_000135067.1 type II secretion system protein GspC -
  LDH51_RS05370 gspD 1077324..1079384 (+) 2061 WP_000498824.1 type II secretion system secretin GspD -
  LDH51_RS05375 pilB/pilB1 1079384..1080877 (+) 1494 WP_000249348.1 type II secretion system ATPase GspE Machinery gene
  LDH51_RS05380 gspF 1080877..1082100 (+) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  LDH51_RS05385 gspG 1082117..1082572 (+) 456 WP_000287130.1 type II secretion system major pseudopilin GspG -
  LDH51_RS05390 gspH 1082576..1083139 (+) 564 WP_001115154.1 type II secretion system minor pseudopilin GspH -
  LDH51_RS05395 gspI 1083136..1083507 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  LDH51_RS05400 gspJ 1083504..1084103 (+) 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
  LDH51_RS05405 gspK 1084106..1085083 (+) 978 WP_000633220.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55204.36 Da        Isoelectric Point: 6.0390

>NTDB_id=593267 LDH51_RS05375 WP_000249348.1 1079384..1080877(+) (pilB/pilB1) [Escherichia coli strain NCCP 15734]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=593267 LDH51_RS05375 WP_000249348.1 1079384..1080877(+) (pilB/pilB1) [Escherichia coli strain NCCP 15734]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCGGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362