Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   K0819_RS01600 Genome accession   NZ_CP080478
Coordinates   336090..336668 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain Vp2015094     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331090..341668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0819_RS01585 (K0819_01595) oxyR 331413..332321 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  K0819_RS01590 (K0819_01600) - 332377..334947 (-) 2571 WP_031430573.1 penicillin-binding protein 1A -
  K0819_RS01595 (K0819_01605) pilM 335090..336106 (+) 1017 WP_109884336.1 type IV pilus biogenesis protein PilM Machinery gene
  K0819_RS01600 (K0819_01610) pilN 336090..336668 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  K0819_RS01605 (K0819_01615) pilO 336661..337254 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  K0819_RS01610 (K0819_01620) pilP 337244..337759 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  K0819_RS01615 (K0819_01625) pilQ 337791..339539 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  K0819_RS01620 (K0819_01630) aroK 339728..340246 (+) 519 WP_005381319.1 shikimate kinase AroK -
  K0819_RS01625 (K0819_01635) aroB 340278..341378 (+) 1101 WP_023622897.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=593173 K0819_RS01600 WP_015297396.1 336090..336668(+) (pilN) [Vibrio parahaemolyticus strain Vp2015094]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=593173 K0819_RS01600 WP_015297396.1 336090..336668(+) (pilN) [Vibrio parahaemolyticus strain Vp2015094]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCTCGGCTAGCAACCATGCTCGATAACATGGAGCGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTCGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTCAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594