Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K0819_RS01595 Genome accession   NZ_CP080478
Coordinates   335090..336106 (+) Length   338 a.a.
NCBI ID   WP_109884336.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain Vp2015094     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330090..341106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0819_RS01580 (K0819_01590) - 330514..331242 (-) 729 WP_015297397.1 redoxin family protein -
  K0819_RS01585 (K0819_01595) oxyR 331413..332321 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  K0819_RS01590 (K0819_01600) - 332377..334947 (-) 2571 WP_031430573.1 penicillin-binding protein 1A -
  K0819_RS01595 (K0819_01605) pilM 335090..336106 (+) 1017 WP_109884336.1 type IV pilus biogenesis protein PilM Machinery gene
  K0819_RS01600 (K0819_01610) pilN 336090..336668 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  K0819_RS01605 (K0819_01615) pilO 336661..337254 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  K0819_RS01610 (K0819_01620) pilP 337244..337759 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  K0819_RS01615 (K0819_01625) pilQ 337791..339539 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  K0819_RS01620 (K0819_01630) aroK 339728..340246 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37834.35 Da        Isoelectric Point: 6.6639

>NTDB_id=593172 K0819_RS01595 WP_109884336.1 335090..336106(+) (pilM) [Vibrio parahaemolyticus strain Vp2015094]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMETSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEEISHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=593172 K0819_RS01595 WP_109884336.1 335090..336106(+) (pilM) [Vibrio parahaemolyticus strain Vp2015094]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGAGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGCTGGCAGAAGAGATCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479