Detailed information NTG0592930

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FBMFHHBO_RS04430
• Genome accession: NZ_CP080452
• Coordinates: 927371..928489 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain MRSN 56
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 922371..933489

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FBMFHHBO_RS04400 (FBMFHHBO_04400)	-	923200..924048 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
FBMFHHBO_RS04405 (FBMFHHBO_04405)	-	924301..924756 (+)	456	WP_000782976.1	bacteriohemerythrin	-
FBMFHHBO_RS04410 (FBMFHHBO_04410)	-	924909..925976 (+)	1068	WP_000092403.1	hypothetical protein	-
FBMFHHBO_RS04415 (FBMFHHBO_04415)	-	925976..926299 (+)	324	WP_000442585.1	RnfH family protein	-
FBMFHHBO_RS04420 (FBMFHHBO_04420)	-	926354..926752 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FBMFHHBO_RS04425 (FBMFHHBO_04425)	fur	926864..927301 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
FBMFHHBO_RS04430 (FBMFHHBO_04430)	pilU	927371..928489 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FBMFHHBO_RS04435 (FBMFHHBO_04435)	pilT	928517..929554 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
FBMFHHBO_RS04440 (FBMFHHBO_04440)	-	929681..930373 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=592930 FBMFHHBO_RS04430 WP_000347036.1 927371..928489(-) (pilU) [Acinetobacter baumannii strain MRSN 56]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=592930 FBMFHHBO_RS04430 WP_000347036.1 927371..928489(-) (pilU) [Acinetobacter baumannii strain MRSN 56]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392