Detailed information NTG0592318

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K0E51_RS03205
• Genome accession: NZ_CP080369
• Coordinates: 708246..709394 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa SG17M
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 703246..714394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K0E51_RS03180 (K0E51_03180)	-	704397..704693 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
K0E51_RS03185 (K0E51_03185)	-	704690..705283 (-)	594	WP_003084544.1	YggT family protein	-
K0E51_RS03190 (K0E51_03190)	proC	705294..706115 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
K0E51_RS03195 (K0E51_03195)	-	706127..706819 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
K0E51_RS03200 (K0E51_03200)	pilT	707034..708068 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
K0E51_RS03205 (K0E51_03205)	pilU	708246..709394 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
K0E51_RS03210 (K0E51_03210)	czcD	709402..710301 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
K0E51_RS03215 (K0E51_03215)	-	710455..710859 (+)	405	WP_003084560.1	NINE protein	-
K0E51_RS03220 (K0E51_03220)	-	711102..712475 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
K0E51_RS03225 (K0E51_03225)	-	712472..713656 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=592318 K0E51_RS03205 WP_003100959.1 708246..709394(+) (pilU) [Pseudomonas aeruginosa SG17M]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=592318 K0E51_RS03205 WP_003100959.1 708246..709394(+) (pilU) [Pseudomonas aeruginosa SG17M]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382