Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PCA10_RS17275 Genome accession   NC_021499
Coordinates   3839472..3840698 (-) Length   408 a.a.
NCBI ID   WP_016493359.1    Uniprot ID   S6AKN8
Organism   Metapseudomonas resinovorans NBRC 106553     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3834472..3845698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCA10_RS17255 (PCA10_34790) - 3834791..3835489 (-) 699 WP_016493355.1 CoA transferase subunit A -
  PCA10_RS17260 (PCA10_34800) - 3835616..3836524 (+) 909 WP_016493356.1 LysR family transcriptional regulator -
  PCA10_RS17265 (PCA10_34810) - 3836576..3838531 (+) 1956 WP_016493357.1 acetoacetate--CoA ligase -
  PCA10_RS17270 (PCA10_34820) - 3838649..3839245 (-) 597 WP_016493358.1 DJ-1/PfpI family protein -
  PCA10_RS17275 (PCA10_34830) pilU 3839472..3840698 (-) 1227 WP_016493359.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCA10_RS17280 (PCA10_34840) - 3840818..3841096 (+) 279 WP_016493360.1 peptidylprolyl isomerase -
  PCA10_RS17285 (PCA10_34850) - 3841166..3841465 (-) 300 WP_016493361.1 hypothetical protein -
  PCA10_RS17290 (PCA10_34860) - 3841546..3841737 (-) 192 WP_016493362.1 hypothetical protein -
  PCA10_RS17295 (PCA10_34870) - 3841908..3842150 (-) 243 WP_016493363.1 hypothetical protein -
  PCA10_RS30985 - 3842386..3842635 (+) 250 Protein_3460 site-specific integrase -
  PCA10_RS17300 (PCA10_34880) - 3842656..3844245 (+) 1590 WP_016493364.1 ABC-F family ATPase -
  PCA10_RS17305 (PCA10_34890) - 3844415..3845023 (-) 609 WP_016493365.1 FMN-dependent NADH-azoreductase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44959.39 Da        Isoelectric Point: 6.1332

>NTDB_id=59203 PCA10_RS17275 WP_016493359.1 3839472..3840698(-) (pilU) [Metapseudomonas resinovorans NBRC 106553]
MDLSAMLKILASQDGSDLYLSTGAPPCAKFNGMLRPLSAEPLKAGDVARIAESIMDAEQRAEFDRELEMNLAISLPNIGR
FRINIFRQRNEVSIVARNIKLDIPRFEDLKLPEVLLKMVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNVGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTSTISDLIKRNEFHSIKEIMEKSRGLGMQTFDQAL
FDLVVEGAIPEDEAIKNADSANNLRLKLKLYRENPATAASAPAAAAAAPVAAAPTPAPSRTEDAASWGLELKLEDLEEDG
PEEPGPRL

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=59203 PCA10_RS17275 WP_016493359.1 3839472..3840698(-) (pilU) [Metapseudomonas resinovorans NBRC 106553]
ATGGACCTCTCCGCGATGCTGAAAATCCTGGCCAGCCAGGATGGTTCCGACCTCTACCTGTCCACGGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCATGCTCCGGCCGCTTTCCGCCGAGCCGCTGAAGGCCGGCGACGTGGCCAGAATCGCCGAATCCA
TCATGGACGCCGAGCAGCGCGCCGAGTTCGACCGTGAGCTGGAGATGAACCTGGCCATCTCGCTGCCCAATATCGGGCGC
TTCCGCATCAATATCTTCCGCCAGCGCAACGAGGTCTCCATCGTCGCGCGGAACATCAAGCTGGACATCCCGCGCTTCGA
GGACCTGAAGCTGCCGGAAGTGCTGCTGAAGATGGTCATGGAAAAACGCGGCCTGGTGCTCTTCGTCGGCGGTACCGGTT
CCGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACTACCGCAATCGCAATGTGGGCGGTCACATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATTTCCACCCTGCACGCCAACAACGCCAACCAGGCCCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGTTGCTCAACGATCTCGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGGGCGGCGGTGGAAGTGCTGCTGGGCACCTCCACTATCAGCGACCTGATCA
AGCGCAACGAATTCCACTCCATCAAGGAGATCATGGAGAAGTCCCGTGGCCTCGGCATGCAGACCTTCGATCAGGCGCTG
TTCGACCTGGTGGTGGAGGGCGCCATTCCCGAAGACGAGGCGATCAAGAACGCCGACTCGGCCAACAACCTGCGCCTGAA
GCTCAAGCTCTACCGCGAAAACCCGGCCACCGCCGCCTCGGCACCCGCCGCTGCCGCAGCGGCCCCCGTGGCCGCCGCCC
CGACTCCGGCGCCCAGCCGCACCGAGGACGCCGCCAGCTGGGGCCTGGAGCTCAAGCTGGAAGACCTGGAGGAGGACGGC
CCGGAAGAGCCGGGGCCGCGATTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AKN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.932

86.765

0.485
  pilU Acinetobacter baylyi ADP1

52.958

87.01

0.461
  pilU Vibrio cholerae strain A1552

51.801

88.48

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.56

95.343

0.368

Multiple sequence alignment